2000
Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA.
Shi H, Djikeng A, Mark T, Wirtz E, Tschudi C, Ullu E. Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA. RNA 2000, 6: 1069-76. PMID: 10917601, PMCID: PMC1369981, DOI: 10.1017/s1355838200000297.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsDNA-Directed RNA PolymerasesDose-Response Relationship, DrugDown-RegulationElectroporationGene ExpressionGenetic EngineeringPhenotypePlasmidsPromoter Regions, GeneticProtein Synthesis InhibitorsProteinsRNA, Double-StrandedTetracyclineTime FactorsTransfectionTrypanosoma brucei bruceiTubulinViral ProteinsConceptsDouble-stranded RNARNA interferenceGenetic interferenceExpression of dsRNAActin mRNAT7 RNA polymerase systemRNA polymerase systemSpecific mRNA degradationStem-loop structureStable cell linesTetracycline-inducible promoterRNAi responseMolecular dissectionMRNA degradationInducible fashionPolymerase systemCellular divisionGene expressionTransfection efficiencyTransient interferenceCell generationCell linesRNAOrganismsInterference
1997
Structure of the Trypanosoma brucei U6 snRNA gene promoter
Nakaar V, Günzl A, Ullu E, Tschudi C. Structure of the Trypanosoma brucei U6 snRNA gene promoter. Molecular And Biochemical Parasitology 1997, 88: 13-23. PMID: 9274863, DOI: 10.1016/s0166-6851(97)00078-9.Peer-Reviewed Original ResearchConceptsU6 snRNA gene promoterSnRNA gene promotersB-boxGene promoterA boxSnRNA gene transcriptionInternal control regionRNA polymerase IIITRNA-like genesU6 transcriptionChromatin organizationTranscription extractRNA genesTranscription unitYeast SaccharomycesYeast systemControl regionPolymerase IIIStart siteGene transcriptionNucleotides upstreamPromoter elementsTrypanosoma bruceiIntragenic sequencesSaturation mutagenesis
1990
Destruction of U2, U4, or U6 small nuclear RNA blocks Trans splicing in trypanosome cells
Tschudi C, Ullu E. Destruction of U2, U4, or U6 small nuclear RNA blocks Trans splicing in trypanosome cells. Cell 1990, 61: 459-466. PMID: 1692263, DOI: 10.1016/0092-8674(90)90527-l.Peer-Reviewed Original ResearchConceptsTrans splicingU snRNAsPre-mRNAU6 small nuclear RNAU4/U6 snRNPsAlpha-tubulin transcriptsSmall nuclear RNAHigh molecular weight RNAMolecular weight RNASite-directed cleavageSL sequenceLeader RNATrypanosome cellsU6 snRNPsNuclear RNAU6 snRNATrypanosoma bruceiSplicing processSplicingWeight RNASnRNAEarly stepsRNAase HRNAIntrons