2000
Cotranscriptional Cap 4 Formation on the Trypanosoma brucei Spliced Leader RNA*
Mair G, Ullu E, Tschudi C. Cotranscriptional Cap 4 Formation on the Trypanosoma brucei Spliced Leader RNA*. Journal Of Biological Chemistry 2000, 275: 28994-28999. PMID: 10880518, DOI: 10.1074/jbc.m004193200.Peer-Reviewed Original ResearchConceptsCap 4 structureSL RNAMRNA cap formationT. brucei cellsSpliced leader RNARNA cap structureSpliced leader sequenceTranscriptional arrestTrypanosomatid protozoaBrucei cellsLeader RNACap 4Cotranscriptional mechanismLeader sequenceTrypanosoma bruceiCap structureRNA transcriptsRibonucleoprotein formationAdenosine residuesPosition 117RNATranscriptsCap formationNucleotidesEukaryotesImport of proteins into the trypanosome nucleus and their distribution at karyokinesis
Marchetti M, Tschudi C, Kwon H, Wolin S, Ullu E. Import of proteins into the trypanosome nucleus and their distribution at karyokinesis. Journal Of Cell Science 2000, 113: 899-906. PMID: 10671379, DOI: 10.1242/jcs.113.5.899.Peer-Reviewed Original ResearchConceptsNuclear localization sequenceMonopartite nuclear localization sequenceBipartite nuclear localization sequenceFusion proteinSpecific nuclear localization sequencesImport of proteinsEarly divergent eukaryoteGreen fluorescent proteinDivergent eukaryotesOrganism's proteinsChromosome segregationBeta-galactosidase reporterTrypanosomatid protozoaProtein homologueTrypanosomal proteinsHistone H2BHistone H2B.Localization sequenceNuclear divisionLa proteinC-terminusTrypanosome nucleusMitotic cellsFluorescent proteinATP synthesis
1998
Trypanosome Capping Enzymes Display a Novel Two-Domain Structure
Silva E, Ullu E, Kobayashi R, Tschudi C. Trypanosome Capping Enzymes Display a Novel Two-Domain Structure. Molecular And Cellular Biology 1998, 18: 4612-4619. PMID: 9671471, PMCID: PMC109047, DOI: 10.1128/mcb.18.8.4612.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCloning, MolecularConserved SequenceCrithidia fasciculataDNA, ProtozoanGuanosine MonophosphateHumansMiceMolecular Sequence DataRecombinant Fusion ProteinsRNA NucleotidyltransferasesSequence Homology, Amino AcidTrypanosoma brucei bruceiConceptsSpliceosomal small nuclear RNAsM7G capCapping enzymeTwo-domain structureG capYeast capping enzymesSmall nuclear RNAPhosphate-binding loopAmino-terminal halfCarboxy-terminal halfGTP-binding proteinsDiphosphate endTrans splicingSignature motifTranscript elongationM7G residueSL sequenceTrypanosomatid protozoaEukaryotic mRNAsLeader RNAGene codesRNA modificationsNuclear RNAOverall identityTrypanosoma brucei
1996
Trans -splicing in trypanosotnatid protozoa
Ullu E, Tschudi C, Gunzl A. Trans -splicing in trypanosotnatid protozoa. 1996, 115-133. DOI: 10.1093/oso/9780199636020.003.0007.Peer-Reviewed Original ResearchTrans splicingSplice siteT. brucei cellsPolycistronic pre-mRNARNA processing reactionsEnd of transcriptsDifferent variant surface glycoproteinsSeparate RNA moleculesVariant surface glycoproteinSL RNAList of organismsSmall RNAsBrucei cellsSL sequenceTrypanosomatid protozoaMature mRNALeader RNAMature speciesPre-mRNATrypanosoma bruceiRNA moleculesProcessing reactionsRNASurface glycoproteinFirst evidence
1995
Accurate Modification of the Trypanosome Spliced Leader Cap Structure in a Homologous Cell-free System (∗)
Ullu E, Tschudi C. Accurate Modification of the Trypanosome Spliced Leader Cap Structure in a Homologous Cell-free System (∗). Journal Of Biological Chemistry 1995, 270: 20365-20369. PMID: 7657609, DOI: 10.1074/jbc.270.35.20365.Peer-Reviewed Original ResearchConceptsSL RNACap structureCell-free systemRibonucleoprotein particleUnusual cap structureSpliced leader sequenceTrans-splicing activityHomologous cell-free systemRNA maturationTrypanosomatid protozoaNucleotide compositionC. fasciculataLeader sequenceTrypanosoma bruceiCell-free extractsT. bruceiT. cruziRNANucleotides 7Vitro reactionBruceiTwo-dimensional thin-layer chromatographyL. amazonensisLeishmania amazonensisCrithidiaAn unusual liaison: Small nuclear and cytoplasmis RNA genes team up with tRNA genes in trypanosomatid protozoa
Nakaar V, Tschudi C, Ullu E. An unusual liaison: Small nuclear and cytoplasmis RNA genes team up with tRNA genes in trypanosomatid protozoa. Trends In Parasitology 1995, 11: 225-228. DOI: 10.1016/0169-4758(95)80085-9.Peer-Reviewed Original ResearchTrypanosomatid protozoaRNA polymerase III transcription unitSequence-specific DNA-binding proteinsCis-acting DNA sequencesBasal transcriptional apparatusDNA-binding proteinsTrans-acting factorsStudy of transcriptionTRNA genesTranscriptional apparatusTranscription unitPromoter structureAccessory factorsRNA polymeraseSmall nuclearDNA sequencesGene teamsCytoplasmic RNAGene expressionEnhancer elementsBiochemical interactionsTransfection technologyRecent insightsProtozoaDiverse set
1994
Trypanosomatid protozoa provide paradigms of eukaryotic biology.
Tschudi C, Ullu E. Trypanosomatid protozoa provide paradigms of eukaryotic biology. Infectious Agents And Disease 1994, 3: 181-6. PMID: 7827786.Peer-Reviewed Original Research