RNA interference in Trypanosoma brucei: cloning of small interfering RNAs provides evidence for retroposon-derived 24-26-nucleotide RNAs.
Djikeng A, Shi H, Tschudi C, Ullu E. RNA interference in Trypanosoma brucei: cloning of small interfering RNAs provides evidence for retroposon-derived 24-26-nucleotide RNAs. RNA 2001, 7: 1522-30. PMID: 11720282, PMCID: PMC1370195.Peer-Reviewed Original ResearchConceptsDouble-stranded RNARNA interferenceGene-specific double-stranded RNAHigh-speed pellet fractionStrand-specific probesSmall interfering RNAsTarget RNA degradationDsRNA resultsHousekeeping functionsTrypanosoma bruceiRNA degradationCellular RNAInterfering RNAsNorthern hybridizationWeight complexesSequence analysisHigh-speed pelletRNALong fragmentSiRNAsPellet fractionEnrichment strategyCloningSupernatant fractionFragments