2015
A designed repeat protein as an affinity capture reagent.
Speltz EB, Brown RS, Hajare HS, Schlieker C, Regan L. A designed repeat protein as an affinity capture reagent. Biochemical Society Transactions 2015, 43: 874-80. PMID: 26517897, PMCID: PMC5683849, DOI: 10.1042/bst20150091.Peer-Reviewed Original ResearchConceptsProtein-peptide interactionsAffinity capture reagentsSupramolecular arraysNovel reagentCapture reagentPractical applicationsReagentsProtein engineeringGeneral utilityAffinity purificationRational fashionProtein interactionsAttractive targetInteractionImportant practical applicationsPurification
2012
A catalytically inactive mutant of the deubiquitylase YOD-1 enhances antigen cross-presentation
Sehrawat S, Koenig PA, Kirak O, Schlieker C, Fankhauser M, Ploegh HL. A catalytically inactive mutant of the deubiquitylase YOD-1 enhances antigen cross-presentation. Blood 2012, 121: 1145-1156. PMID: 23243279, PMCID: PMC3575758, DOI: 10.1182/blood-2012-08-447409.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAnimalsAntigen-Presenting CellsATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersBrefeldin ACD8-Positive T-LymphocytesCross-PrimingFemaleHydrogen-Ion ConcentrationImmunizationMaleMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicMutation, MissenseOvalbuminPeptide FragmentsRhadinovirusUbiquitin ThiolesteraseConceptsAntigen presenting cellsT cellsPresent exogenous antigensRecombinant herpes virusT cell responsesExogenous antigensPresenting cellsSIINFEKL peptideRecombinant influenzaAntigen processingInclusion of inhibitorsEnhanced expansionMHV-68AntigenImmunizationInfectionVirusCellsCrosspresentationImproved controlInfluenzaMiceSIINFEKL