2019
Methodologies to monitor protein turnover at the inner nuclear membrane
Tsai PL, Zhao C, Schlieker C. Methodologies to monitor protein turnover at the inner nuclear membrane. Methods In Enzymology 2019, 619: 47-69. PMID: 30910029, PMCID: PMC6457266, DOI: 10.1016/bs.mie.2018.12.033.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell FractionationCell LineCell NucleusFlow CytometryFluorescent Antibody TechniqueGreen Fluorescent ProteinsHumansNuclear EnvelopeOptical ImagingReceptors, Cytoplasmic and NuclearConceptsLamin B receptorNuclear envelopeInner nuclear membrane proteinProtein turnoverProtein quality control pathwaysNuclear membrane proteinsQuality control pathwaysProtein turnover machineryHuman congenital disordersInner nuclear membraneSubcellular fractionation methodMammalian nuclear envelopeLive-cell imagingC-terminal truncationsNuclear laminaMembrane proteinsModel substrateBiochemical approachesNuclear compartmentActivity essentialControl pathwaysNuclear membraneRapid turnoverCholesterol biosynthesisCell imaging
2016
The Lamin B receptor is essential for cholesterol synthesis and perturbed by disease-causing mutations
Tsai PL, Zhao C, Turner E, Schlieker C. The Lamin B receptor is essential for cholesterol synthesis and perturbed by disease-causing mutations. ELife 2016, 5: e16011. PMID: 27336722, PMCID: PMC4951196, DOI: 10.7554/elife.16011.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedCholesterolHumansMutationOsteochondrodysplasiasPelger-Huet AnomalyReceptors, Cytoplasmic and NuclearConceptsLamin B receptorInner nuclear membraneNuclear membraneHuman lamin B receptorPolytopic membrane proteinsGreenberg skeletal dysplasiaProtein turnover mechanismsHigher eukaryotesDisease-causing mutationsHuman cell linesNuclear laminaMutant proteinsMembrane proteinsMutant derivativesB receptorCholesterol auxotrophyTurnover mechanismsDisease-causing variantsPoint mutationsCholesterol synthesisEnzymatic activityCofactor NADPHFunction mechanismCell linesRapid degradation