2024
Protein folding and quality control during nuclear transport
Mallik S, Poch D, Burick S, Schlieker C. Protein folding and quality control during nuclear transport. Current Opinion In Cell Biology 2024, 90: 102407. PMID: 39142062, DOI: 10.1016/j.ceb.2024.102407.Peer-Reviewed Original ResearchProtein foldingNuclear transportCo-translational transportProtein-folding environmentAggregation of folding intermediatesProtein quality controlNuclear import machineryNuclear pore complexFolding environmentImport machineryNuclear importCytosolic aggregatesContext of neurological disordersNuclear compartmentPore complexProtein synthesisProteinQuality controlFoldingBarrier functionEvolution of transport systemsKaryopherinNucleoporinsCompartmentTemporal coordination
2019
Methodologies to monitor protein turnover at the inner nuclear membrane
Tsai PL, Zhao C, Schlieker C. Methodologies to monitor protein turnover at the inner nuclear membrane. Methods In Enzymology 2019, 619: 47-69. PMID: 30910029, PMCID: PMC6457266, DOI: 10.1016/bs.mie.2018.12.033.Peer-Reviewed Original ResearchConceptsLamin B receptorNuclear envelopeInner nuclear membrane proteinProtein turnoverProtein quality control pathwaysNuclear membrane proteinsQuality control pathwaysProtein turnover machineryHuman congenital disordersInner nuclear membraneSubcellular fractionation methodMammalian nuclear envelopeLive-cell imagingC-terminal truncationsNuclear laminaMembrane proteinsModel substrateBiochemical approachesNuclear compartmentActivity essentialControl pathwaysNuclear membraneRapid turnoverCholesterol biosynthesisCell imaging