2012
Targeted Delivery of Chemotherapy Agents Using a Liver Cancer-Specific Aptamer
Meng L, Yang L, Zhao X, Zhang L, Zhu H, Liu C, Tan W. Targeted Delivery of Chemotherapy Agents Using a Liver Cancer-Specific Aptamer. PLOS ONE 2012, 7: e33434. PMID: 22558072, PMCID: PMC3338807, DOI: 10.1371/journal.pone.0033434.Peer-Reviewed Original ResearchConceptsAptamer-drug conjugatesCancer-specific aptamersAntitumor agent doxorubicinDOX conjugatesIntercalation methodAptamerCell viability testTargeted deliveryConjugatesCancer cellsTarget proteinsAgent doxorubicinPotential candidateCell linesDOXEfficiency of chemotherapyLiver cancer cellsNonspecific uptakeTarget specificityAvailable aptamersCarcinoma cell linesChemotherapy agentsHepatocellular carcinoma cell linesHuman hepatocellular carcinoma cell linePromising method
2011
Development of optimized AAV3 serotype vectors: mechanism of high-efficiency transduction of human liver cancer cells
Cheng B, Ling C, Dai Y, Lu Y, Glushakova L, Gee S, McGoogan K, Aslanidi G, Park M, Stacpoole P, Siemann D, Liu C, Srivastava A, Ling C. Development of optimized AAV3 serotype vectors: mechanism of high-efficiency transduction of human liver cancer cells. Gene Therapy 2011, 19: 375-384. PMID: 21776025, PMCID: PMC3519243, DOI: 10.1038/gt.2011.105.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBreast NeoplasmsCarcinoma, HepatocellularCell Line, TumorDependovirusFemaleGenetic VectorsHumansLiver NeoplasmsMiceMutagenesis, Site-DirectedProteasome InhibitorsProtein Structure, TertiaryProto-Oncogene Proteins c-metTransduction, GeneticTransplantation, HeterologousTyrosineVirus IntegrationConceptsHigh-efficiency transductionAAV3 vectorsTransduction efficiencySerotype vectorsSurface-exposed tyrosinePotential gene therapyHuman hepatocyte growth factor receptorHuman liver cancer cellsGene therapyLiver cancer cellsTransgene expressionVector entryAAV3 capsidsDirect intratumoral injectionAAV3Tail vein injectionLiver tumor cellsIntratumoral injectionCancer cellsEfficiencyAdenoVectorIntracellular kinase domainVirus serotypesSurvivin inhibition is critical for Bcl‐2 family inhibitor, ABT‐263 to induce apoptosis in liver cancer cells
zhao X, Ogunwobi O, Nelson D, Liu C. Survivin inhibition is critical for Bcl‐2 family inhibitor, ABT‐263 to induce apoptosis in liver cancer cells. The FASEB Journal 2011, 25: 563.13-563.13. DOI: 10.1096/fasebj.25.1_supplement.563.13.Peer-Reviewed Original ResearchYM-155Liver cancer cellsABT-263HCC cellsProtein expressionCancer cellsABT-263 treatmentNormal hepatocytesMechanism of actionHCC cell apoptosisBcl-2 inhibitorsSurvivin protein expressionBcl-2 family inhibitorsHCC managementSignificant apoptotic effectHigh dosesLow dosesSurvivin expressionNovel Bcl-2 inhibitorSurvivin inhibitionSurvivin inhibitorChemotherapeutic alternativesInhibitory effectCell apoptosisSurvivin siRNA
2009
AAV3-mediated transfer and expression of the pyruvate dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of human liver cancer cells
Glushakova L, Lisankie M, Eruslanov E, Ojano-Dirain C, Zolotukhin I, Liu C, Srivastava A, Stacpoole P. AAV3-mediated transfer and expression of the pyruvate dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of human liver cancer cells. Molecular Genetics And Metabolism 2009, 98: 289-299. PMID: 19586787, PMCID: PMC2779054, DOI: 10.1016/j.ymgme.2009.05.010.Peer-Reviewed Original ResearchConceptsPyruvate dehydrogenase complexSubunit geneReporter geneWarburg effectCell deathCancer cellsGreen fluorescent reporter geneLiver cancer cellsAlpha-subunit geneFluorescent reporter geneHuman hepatoblastomaE1alpha subunit geneHuH-6 cellsApoptotic cell deathProtein expressionMitochondrial oxidative metabolismHuman alpha-fetoprotein promoterMitochondrial respiratory chain activityBeta-actin promoterRespiratory chain activityChicken beta-actin promoterReactive oxygen speciesExpression systemDehydrogenase complexMetabolic remodeling
2008
Identification of Liver Cancer-Specific Aptamers Using Whole Live Cells
Shangguan D, Meng L, Cao Z, Xiao Z, Fang X, Li Y, Cardona D, Witek R, Liu C, Tan W. Identification of Liver Cancer-Specific Aptamers Using Whole Live Cells. Analytical Chemistry 2008, 80: 721-728. PMID: 18177018, DOI: 10.1021/ac701962v.Peer-Reviewed Original ResearchConceptsLiver cancerLiver cancer cellsEarly diagnosisCell linesBALB/cJ miceCancer cellsSurgical resectionClinical outcomesLiver cell lineEffective treatmentMouse modelWhole live cellsDeadly cancerCurrent studyCancer-specific aptamersTumor selectionBasic mechanism studiesCancerMost cancersBNL CLCancer early diagnosisCell-SELEX methodNoncancer cell linesDiagnosisCancer recognition