2023
Leptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia Pathogenesis
Ozmen A, Nwabuobi C, Tang Z, Guo X, Larsen K, Guller S, Blas J, Moore M, Kayisli U, Lockwood C, Guzeloglu-Kayisli O. Leptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia Pathogenesis. International Journal Of Molecular Sciences 2023, 25: 135. PMID: 38203306, PMCID: PMC10778808, DOI: 10.3390/ijms25010135.Peer-Reviewed Original ResearchMeSH KeywordsFemaleHumansInterleukin-6LeptinNF-kappa BPlacentaPre-EclampsiaPregnancySTAT5 Transcription FactorConceptsIL-6 expressionHofbauer cellsP65 NF-κBIL-6NF-κBLeptin levelsPreeclampsia pathogenesisElevated serum interleukin-6 levelsSerum interleukin-6 levelsERK1/2 MAPKInterleukin-6 levelsPro-inflammatory phenotypeAnti-inflammatory phenotypeLeptin receptor expressionIL-6 productionPathogenesis of PEPlacental leptin productionPhosphorylation levelsERK1/2 MAPK inhibitorIL-6 regulationConcentration-dependent mannerPE patientsHuman pregnancySecond trimesterLeptin concentrations
2008
Placental expression of ceruloplasmin in pregnancies complicated by severe preeclampsia
Guller S, Buhimschi CS, Y Y, Huang T, Yang L, Kuczynski E, Zambrano E, Lockwood CJ, Buhimschi IA. Placental expression of ceruloplasmin in pregnancies complicated by severe preeclampsia. Laboratory Investigation 2008, 88: 1057-1067. PMID: 18679377, PMCID: PMC2682720, DOI: 10.1038/labinvest.2008.74.Peer-Reviewed Original ResearchConceptsPlasminogen activator inhibitor-1Severe preeclampsiaReperfusion injurySFlt-1Soluble fms-like tyrosine kinase-1Fms-like tyrosine kinase-1Ischemia/reperfusion injuryLife-threatening syndromeSubsequent reperfusion injuryTerm control groupPathophysiology of preeclampsiaTyrosine kinase-1Activator inhibitor-1Release of factorsSignificant increasePresence of mRNAUpregulation of mRNACeruloplasmin mRNAPlacental damagePlacental factorsMicroarray gene profilingEndothelium dysfunctionPE placentasQuantitative real-time PCRIntervillous spacePreeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells
Lockwood CJ, Yen CF, Basar M, Kayisli UA, Martel M, Buhimschi I, Buhimschi C, Huang SJ, Krikun G, Schatz F. Preeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells. American Journal Of Pathology 2008, 172: 1571-1579. PMID: 18467705, PMCID: PMC2408417, DOI: 10.2353/ajpath.2008.070629.Peer-Reviewed Original ResearchConceptsIL-6 levelsIL-6Decidual cellsGestational age-matched control placentasElevated IL-6 levelsIL-6 mRNA levelsCommon pregnancy disorderGenesis of preeclampsiaUteroplacental blood flowFetal-placental unitInterleukin-6 levelsPathogenesis of preeclampsiaPro-inflammatory cytokinesEndothelial cell dysfunctionInterleukin-6 expressionHuman decidual cellsReal-time quantitative RT-PCRTime quantitative RT-PCRQuantitative RT-PCRFetal morbidityDecidual macrophagesPreeclamptic womenSystemic inflammationPregnancy disordersControl placentasDecidual Cell‐expressed Tissue Factor in Human Pregnancy and Its Involvement in Hemostasis and Preeclampsia‐related Angiogenesis
Lockwood CJ, Krikun G, Caze R, Rahman M, Buchwalder LF, Schatz F. Decidual Cell‐expressed Tissue Factor in Human Pregnancy and Its Involvement in Hemostasis and Preeclampsia‐related Angiogenesis. Annals Of The New York Academy Of Sciences 2008, 1127: 67-72. PMID: 18443332, DOI: 10.1196/annals.1434.013.Peer-Reviewed Original ResearchConceptsDecidual cell-expressed tissue factorUteroplacental blood flowVascular endothelial growth factorTissue factorDecidual cellsBlood flowFirst trimester decidual cellsGrowth factorPlacental growth factorProtease-activated receptorsSFlt-1 expressionEndothelial growth factorPredominant cell typeMaternal thrombophiliaMaternal morbidityDecidual hemorrhageSpiral arteriesHuman pregnancySFlt-1Trophoblast invasionVascular remodelingPreeclampsiaVEGF expressionExcess thrombinAngiogenic effectsMatrix Metalloproteinase 9 (MMP9) Expression in Preeclamptic Decidua and MMP9 Induction by Tumor Necrosis Factor Alpha and Interleukin 1 Beta in Human First Trimester Decidual Cells1
Lockwood CJ, Oner C, Uz YH, Kayisli UA, Huang SJ, Buchwalder LF, Murk W, Funai EF, Schatz F. Matrix Metalloproteinase 9 (MMP9) Expression in Preeclamptic Decidua and MMP9 Induction by Tumor Necrosis Factor Alpha and Interleukin 1 Beta in Human First Trimester Decidual Cells1. Biology Of Reproduction 2008, 78: 1064-1072. PMID: 18276934, PMCID: PMC3045968, DOI: 10.1095/biolreprod.107.063743.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCase-Control StudiesCells, CulturedDeciduaDNA PrimersEnzyme InductionFemaleHumansImmunohistochemistryInterleukin-1betaMatrix Metalloproteinase 2Matrix Metalloproteinase 9Pre-EclampsiaPregnancyPregnancy Trimester, FirstReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTissue Inhibitor of Metalloproteinase-1Tissue Inhibitor of Metalloproteinase-2Tumor Necrosis Factor-alphaConceptsTumor necrosis factor alphaNecrosis factor alphaInterleukin-1 betaMedroxyprogesterone acetateFactor alphaDecidual cellsMMP9 levelsExtravillous trophoblastsEVT invasionFirst trimester decidual cellsAge-matched control womenMatrix metalloproteinase-9 expressionUteroplacental blood flowLevels of MMP9Metalloproteinase-9 expressionMatrix metalloproteinases 1Matrix metalloproteinases 2Levels of MMP2Decidual extracellular matrixConcentration-dependent increaseSubstrate gel zymographyPreeclamptic deciduaQuantitative RT-PCRInterstitial trophoblastSpiral arteries