2023
Molecular mechanism of hyperactivation conferred by a truncation of TRPA1
Bali A, Schaefer S, Trier I, Zhang A, Kabeche L, Paulsen C. Molecular mechanism of hyperactivation conferred by a truncation of TRPA1. Nature Communications 2023, 14: 2867. PMID: 37208332, PMCID: PMC10199097, DOI: 10.1038/s41467-023-38542-1.Peer-Reviewed Original ResearchConceptsChannel sensitizationPlasma membraneHeterologous cellsGenetic analysisMolecular mechanismsBiochemical assaysHeteromeric channelsNonsense mutationPhysiological impactMutantsAgonist sensitivityCalcium permeabilityEnergetic barrierSubunitsTRPA1MutationsTractable mechanismMechanismHyperactivationMembraneCellsActivationAssaysGatingTruncation
2020
Irritant-evoked activation and calcium modulation of the TRPA1 receptor
Zhao J, Lin King JV, Paulsen CE, Cheng Y, Julius D. Irritant-evoked activation and calcium modulation of the TRPA1 receptor. Nature 2020, 585: 141-145. PMID: 32641835, PMCID: PMC7483980, DOI: 10.1038/s41586-020-2480-9.Peer-Reviewed Original ResearchConceptsPrimary afferent nerve fibersTRP channel subtypesAfferent nerve fibersIon channel TRPA1Inflammatory painIrritant receptorsMetabotropic receptorsTRPA1 receptorsCytoplasmic second messengersNerve fibersChannel subtypesCalcium permeabilityCalcium modulationTRPA1 regulationTRPA1Environmental toxicantsFunctional couplingReceptorsPainItchExogenous agentsAttractive targetIrritantsActivationSecond messenger
2016
Molecular Basis for Redox Activation of Epidermal Growth Factor Receptor Kinase
Truong TH, Ung PM, Palde PB, Paulsen CE, Schlessinger A, Carroll KS. Molecular Basis for Redox Activation of Epidermal Growth Factor Receptor Kinase. Cell Chemical Biology 2016, 23: 837-848. PMID: 27427230, PMCID: PMC4958504, DOI: 10.1016/j.chembiol.2016.05.017.Peer-Reviewed Original ResearchConceptsEpidermal growth factor receptorEpidermal growth factor receptor kinaseGrowth factor receptor kinaseDetailed functional analysisNew electrostatic interactionsFirst detailed functional analysisKinase regulationS-sulfenylationRedox biologyReceptor kinaseCatalytic loopGrowth factor receptorMolecular basisChronic oxidative stressKinase activityFunctional analysisCatalytic importanceRedox activationFactor receptorMajor classesOxidative stressCatalytic efficiencyData highlightMolecular dynamics simulationsActivation