2022
The role of FYCO1-dependent autophagy in lens fiber cell differentiation
Khan SY, Ali M, Kabir F, Na CH, Delannoy M, Ma Y, Qiu C, Costello MJ, Hejtmancik JF, Riazuddin SA. The role of FYCO1-dependent autophagy in lens fiber cell differentiation. Autophagy 2022, 18: 2198-2215. PMID: 35343376, PMCID: PMC9397473, DOI: 10.1080/15548627.2022.2025570.Peer-Reviewed Original ResearchConceptsAutophagic vesiclesLens fiber cell differentiationMouse lensesAutophagic fluxFiber cell differentiationHuman embryonic stem cellsCoiled-coil domainOrganelle-free zoneEmbryonic stem cellsHuman lens epithelial cellsAutophagy-associated genesSingle guide RNAsQuantitative real-time PCRLens epithelial cellsAdaptor proteinRNA-seqGuide RNARNA sequencingCellular organellesLens morphogenesisCataract phenotypeWild typeFYCO1Cell differentiationEndoplasmic reticulum
2018
Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells
Ali M, Khan SY, Vasanth S, Ahmed MR, Chen R, Na CH, Thomson JJ, Qiu C, Gottsch JD, Riazuddin SA. Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells. Investigative Ophthalmology & Visual Science 2018, 59: 2437-2444. PMID: 29847650, PMCID: PMC5957521, DOI: 10.1167/iovs.17-22927.Peer-Reviewed Original ResearchMeSH KeywordsAgedCell DifferentiationCells, CulturedCryopreservationEmbryonic Stem CellsEndothelium, CornealFlow CytometryGene Expression ProfilingGenetic MarkersHumansImmunohistochemistryInduced Pluripotent Stem CellsLeukocytes, MononuclearMaleMass SpectrometryMicroscopy, Phase-ContrastMiddle AgedNeural CrestProteomeReal-Time Polymerase Chain ReactionConceptsNeural crest cellsProteome sequencingProteome profilingCorneal endothelial cellsExpression of pluripotentQuantitative real-time PCRPluripotent stem cellsMolecular architectureCrest cellsEndothelial cellsProteomeReal-time PCRPluripotency markersHuman corneal endotheliumStem cellsPhase contrast microscopyExpression levelsProteinIPSCsSequencingCellsProfilingFirst reportContrast microscopyHigh levels