Loss of T cell responses following long-term cryopreservation
Owen RE, Sinclair E, Emu B, Heitman JW, Hirschkorn DF, Epling CL, Tan QX, Custer B, Harris JM, Jacobson MA, McCune JM, Martin JN, Hecht FM, Deeks SG, Norris PJ. Loss of T cell responses following long-term cryopreservation. Journal Of Immunological Methods 2007, 326: 93-115. PMID: 17707394, PMCID: PMC2065759, DOI: 10.1016/j.jim.2007.07.012.Peer-Reviewed Original ResearchConceptsT cell IFN-gamma responsesT cell responsesHuman immunodeficiency virusIFN-gamma responsesPeripheral blood mononuclear cellsCell responsesLong-term cryopreservationPeptide poolsHIV-negative individualsBlood mononuclear cellsLoss of responseT cell apoptosisPhenotypic marker expressionDecrement of responseOvernight restingEffector cellsImmunodeficiency virusExogenous antigensMononuclear cellsShort-term cryopreservationHIV peptidesFuture vaccinesImpact of cryopreservationPathogenesis studiesMarker expression