2009
Hepatitis C Virus NS2 Protein Contributes to Virus Particle Assembly via Opposing Epistatic Interactions with the E1-E2 Glycoprotein and NS3-NS4A Enzyme Complexes
Phan T, Beran RK, Peters C, Lorenz IC, Lindenbach BD. Hepatitis C Virus NS2 Protein Contributes to Virus Particle Assembly via Opposing Epistatic Interactions with the E1-E2 Glycoprotein and NS3-NS4A Enzyme Complexes. Journal Of Virology 2009, 83: 8379-8395. PMID: 19515772, PMCID: PMC2738163, DOI: 10.1128/jvi.00891-09.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino Acid SubstitutionCarrier ProteinsHepacivirusHumansIntracellular Signaling Peptides and ProteinsModels, MolecularMolecular Sequence DataMutation, MissenseProtein BindingProtein Interaction MappingSuppression, GeneticViral Envelope ProteinsViral Nonstructural ProteinsViral ProteinsVirus AssemblyConceptsHepatitis C Virus NS2 ProteinVirus particle assemblyNS2 proteinVirus assemblyReverse genetic analysisForward genetic selectionSecond-site mutationsNS2 mutantsSuppressor mutationsRNA bindingEpistatic interactionsBiochemical experimentsGenetic analysisEnzyme complexSevere defectsNonstructural proteinsGenetic selectionParticle assemblyRNA replicationCell culture systemMutationsNS3-NS4AProteinMutantsMutual exclusivity
2005
Unravelling hepatitis C virus replication from genome to function
Lindenbach BD, Rice CM. Unravelling hepatitis C virus replication from genome to function. Nature 2005, 436: 933-938. PMID: 16107832, DOI: 10.1038/nature04077.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGene Expression Regulation, ViralGenome, ViralHepacivirusHumansRNA, ViralViral ProteinsVirus Replication
1998
Noncytopathic Sindbis virus RNA vectors for heterologous gene expression
Agapov E, Frolov I, Lindenbach B, Prágai B, Schlesinger S, Rice C. Noncytopathic Sindbis virus RNA vectors for heterologous gene expression. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 12989-12994. PMID: 9789028, PMCID: PMC23682, DOI: 10.1073/pnas.95.22.12989.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta-GalactosidaseCell LineCloning, MolecularCricetinaeDNA-Directed RNA PolymerasesElectroporationGenes, ReporterGenetic VectorsGreen Fluorescent ProteinsKidneyLuminescent ProteinsPlasmidsRecombinant ProteinsRepliconRNA, ViralSindbis VirusTranscription, GeneticTransfectionViral ProteinsConceptsForeign gene expressionGene expressionLong-term foreign gene expressionSindbis vectorsHeterologous gene expressionDefective interfering genomesVirus-encoded genesHost protein synthesisVertebrate cellsHeterologous genesRecombinant Sindbis virusForeign genesDrug puromycinCellular metabolismProtein synthesisDNA transfectionRNA vectorSubgenomic transcriptsForeign proteinsRapid expressionBHK cellsGenesRepliconSindbis virusDramatic inhibition
1994
Processing in the hepatitis C virus E2-NS2 region: identification of p7 and two distinct E2-specific products with different C termini
Lin C, Lindenbach B, Prágai B, McCourt D, Rice C. Processing in the hepatitis C virus E2-NS2 region: identification of p7 and two distinct E2-specific products with different C termini. Journal Of Virology 1994, 68: 5063-5073. PMID: 7518529, PMCID: PMC236449, DOI: 10.1128/jvi.68.8.5063-5073.1994.Peer-Reviewed Original Research