Noncytopathic Sindbis virus RNA vectors for heterologous gene expression
Agapov E, Frolov I, Lindenbach B, Prágai B, Schlesinger S, Rice C. Noncytopathic Sindbis virus RNA vectors for heterologous gene expression. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 12989-12994. PMID: 9789028, PMCID: PMC23682, DOI: 10.1073/pnas.95.22.12989.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta-GalactosidaseCell LineCloning, MolecularCricetinaeDNA-Directed RNA PolymerasesElectroporationGenes, ReporterGenetic VectorsGreen Fluorescent ProteinsKidneyLuminescent ProteinsPlasmidsRecombinant ProteinsRepliconRNA, ViralSindbis VirusTranscription, GeneticTransfectionViral ProteinsConceptsForeign gene expressionGene expressionLong-term foreign gene expressionSindbis vectorsHeterologous gene expressionDefective interfering genomesVirus-encoded genesHost protein synthesisVertebrate cellsHeterologous genesRecombinant Sindbis virusForeign genesDrug puromycinCellular metabolismProtein synthesisDNA transfectionRNA vectorSubgenomic transcriptsForeign proteinsRapid expressionBHK cellsGenesRepliconSindbis virusDramatic inhibition