2010
The Acidic Domain of Hepatitis C Virus NS4A Contributes to RNA Replication and Virus Particle Assembly
Phan T, Kohlway A, Dimberu P, Pyle AM, Lindenbach BD. The Acidic Domain of Hepatitis C Virus NS4A Contributes to RNA Replication and Virus Particle Assembly. Journal Of Virology 2010, 85: 1193-1204. PMID: 21047963, PMCID: PMC3020511, DOI: 10.1128/jvi.01889-10.Peer-Reviewed Original ResearchConceptsVirus particle assemblyAcidic domainGenome replicationAssembly defectsEnzyme complexSevere defectsMembrane-bound enzyme complexRNA replicationTerminal acidic domainNS3-4AAlanine-scanning mutagenesisSecond-site mutationsViral genome replicationHepatitis C virus (HCV) NS3-4AGenetic interactionsRNA helicaseParticle assemblyReplication defectScanning mutagenesisMutant formsVirus assemblySerine proteasesEnzymatic activityATPase activityMutants
2009
Hepatitis C Virus NS2 Protein Contributes to Virus Particle Assembly via Opposing Epistatic Interactions with the E1-E2 Glycoprotein and NS3-NS4A Enzyme Complexes
Phan T, Beran RK, Peters C, Lorenz IC, Lindenbach BD. Hepatitis C Virus NS2 Protein Contributes to Virus Particle Assembly via Opposing Epistatic Interactions with the E1-E2 Glycoprotein and NS3-NS4A Enzyme Complexes. Journal Of Virology 2009, 83: 8379-8395. PMID: 19515772, PMCID: PMC2738163, DOI: 10.1128/jvi.00891-09.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino Acid SubstitutionCarrier ProteinsHepacivirusHumansIntracellular Signaling Peptides and ProteinsModels, MolecularMolecular Sequence DataMutation, MissenseProtein BindingProtein Interaction MappingSuppression, GeneticViral Envelope ProteinsViral Nonstructural ProteinsViral ProteinsVirus AssemblyConceptsHepatitis C Virus NS2 ProteinVirus particle assemblyNS2 proteinVirus assemblyReverse genetic analysisForward genetic selectionSecond-site mutationsNS2 mutantsSuppressor mutationsRNA bindingEpistatic interactionsBiochemical experimentsGenetic analysisEnzyme complexSevere defectsNonstructural proteinsGenetic selectionParticle assemblyRNA replicationCell culture systemMutationsNS3-NS4AProteinMutantsMutual exclusivity