Reduced Hypoxia-Related Genes in Porcine Limbs in Ex Vivo Hypothermic Perfusion Versus Cold Storage
Krezdorn N, Sakthivel D, Turk M, Aycart M, Tasigiorgos S, Bueno E, Sinha I, Pomahac B. Reduced Hypoxia-Related Genes in Porcine Limbs in Ex Vivo Hypothermic Perfusion Versus Cold Storage. Journal Of Surgical Research 2018, 232: 137-145. PMID: 30463709, DOI: 10.1016/j.jss.2018.05.067.Peer-Reviewed Original ResearchConceptsStatic cold storagePerfusion groupIschemia-reperfusion injuryPCR gene arrayPorcine limbsMuscle fiber disruptionIschemic injuryPerfadex solutionPerfused limbMachine perfusionHypothermic perfusionHistological changesPreservation modalityMuscle biopsyLimb replantationPerfusionICE groupLess activationPorcine forelimbDownregulation of genesMolecular changesLimbFiber disruptionExpression levelsReplantation