2016
Parallel evolution of male germline epigenetic poising and somatic development in animals
Lesch BJ, Silber SJ, McCarrey JR, Page DC. Parallel evolution of male germline epigenetic poising and somatic development in animals. Nature Genetics 2016, 48: 888-894. PMID: 27294618, DOI: 10.1038/ng.3591.Peer-Reviewed Original Research
2014
Poised chromatin in the mammalian germ line
Lesch BJ, Page DC. Poised chromatin in the mammalian germ line. Development 2014, 141: 3619-3626. PMID: 25249456, PMCID: PMC4197577, DOI: 10.1242/dev.113027.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsMammalian germ lineGerm cell identityCell identityGerm lineMammalian germ cellsEmbryonic stem cellsChromatin stateEpigenetic inheritanceHistone modificationsRegulatory genesGene activationDNA methylationEpigenetic featuresChromatinGerm cellsStem cellsSomatic developmentGenesPromoterFetal stageTotipotencyCellsMeiosisGametogenesisRepression
2013
A set of genes critical to development is epigenetically poised in mouse germ cells from fetal stages through completion of meiosis
Lesch BJ, Dokshin GA, Young RA, McCarrey JR, Page DC. A set of genes critical to development is epigenetically poised in mouse germ cells from fetal stages through completion of meiosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 16061-16066. PMID: 24043772, PMCID: PMC3791702, DOI: 10.1073/pnas.1315204110.Peer-Reviewed Original ResearchConceptsSet of genesGerm cellsPostmeiotic male germ cellsGerm line nucleiSexual differentiationRNA-seq analysisFemale germ cellsSubset of genesEmbryonic stem cellsMale germ cellsRegulators of differentiationEnd of meiosisCompletion of meiosisMouse germ cellsTotipotent zygoteDifferentiated gametesMulticellular organismsGamete precursorsDevelopmental genesChIP-seqGerm lineGamete unionExtraembryonic tissuesMeiotic initiationPostmeiotic cellsThe Ligand Binding Domain of GCNF Is Not Required for Repression of Pluripotency Genes in Mouse Fetal Ovarian Germ Cells
Okumura LM, Lesch BJ, Page DC. The Ligand Binding Domain of GCNF Is Not Required for Repression of Pluripotency Genes in Mouse Fetal Ovarian Germ Cells. PLOS ONE 2013, 8: e66062. PMID: 23762465, PMCID: PMC3676325, DOI: 10.1371/journal.pone.0066062.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDown-RegulationExonsFemaleFetusGene Expression Regulation, DevelopmentalGene TargetingGerm CellsHomeodomain ProteinsLigandsMeiosisMiceMice, Inbred C57BLMice, Mutant StrainsMutationNanog Homeobox ProteinNuclear Receptor Subfamily 6, Group A, Member 1Octamer Transcription Factor-3OogenesisOvaryPhenotypePluripotent Stem CellsProtein BindingProtein Structure, TertiarySequence DeletionStructure-Activity RelationshipConceptsFetal ovarian germ cellsOvarian germ cellsPluripotency genesSomatic cellsGerm cellsSilencing of Oct4Initiation of meiosisEmbryonic stem cellsLigand binding domainsGCNF geneEmbryonic day 14.5Tamoxifen-inducible CreDifferent developmental timepointsBinding domainsDifferentiated stateGCNFDevelopmental timepointsNanogGenesConditional ablationStem cellsDay 14.5CellsExpressionE8.0