2024
Selective utilization of glucose metabolism guides mammalian gastrulation
Cao D, Bergmann J, Zhong L, Hemalatha A, Dingare C, Jensen T, Cox A, Greco V, Steventon B, Sozen B. Selective utilization of glucose metabolism guides mammalian gastrulation. Nature 2024, 634: 919-928. PMID: 39415005, PMCID: PMC11499262, DOI: 10.1038/s41586-024-08044-1.Peer-Reviewed Original ResearchConceptsCellular metabolismMammalian gastrulationHexosamine biosynthetic pathwayTranscription factor networksCellular signaling pathwaysSignaling morphogensGlucose metabolismCellular programmeBiosynthetic pathwayFate acquisitionCell fateHousekeeping natureGenetic mechanismsMesoderm migrationFactor networksERK activationExpression patternsSignaling pathwayDevelopmental processesStem cell modelCell typesSpecialized functionsDevelopmental contextMammalian embryosMouse embryos
2021
Machine learning-assisted high-content analysis of pluripotent stem cell-derived embryos in vitro
Guo J, Wang P, Sozen B, Qiu H, Zhu Y, Zhang X, Ming J, Zernicka-Goetz M, Na J. Machine learning-assisted high-content analysis of pluripotent stem cell-derived embryos in vitro. Stem Cell Reports 2021, 16: 1331-1346. PMID: 33891867, PMCID: PMC8185434, DOI: 10.1016/j.stemcr.2021.03.018.Peer-Reviewed Original Research
2020
Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro
Amadei G, Lau K, De Jonghe J, Gantner C, Sozen B, Chan C, Zhu M, Kyprianou C, Hollfelder F, Zernicka-Goetz M. Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro. Developmental Cell 2020, 56: 366-382.e9. PMID: 33378662, PMCID: PMC7883308, DOI: 10.1016/j.devcel.2020.12.004.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsTrophoblast stem cellsDevelopment of mouse embryosStem cellsXEN cellsTranscription factor GATA4Extra-embryonic mesodermTs cellsExpressed GATA4Mouse embryosAnterior signaling centerAnterior-posterior patterningDevelopmental potentialExtra-embryonic endodermDefinitive endodermMouse gastrulaGATA4Morphogenetic eventsSignaling centersMorphogenic eventsEndodermal fateEmbryosCellsGastrulationEmbryo-like structuresThe dynamics of morphogenesis in stem cell-based embryology: Novel insights for symmetry breaking
Sozen B, Cornwall-Scoones J, Zernicka-Goetz M. The dynamics of morphogenesis in stem cell-based embryology: Novel insights for symmetry breaking. Developmental Biology 2020, 474: 82-90. PMID: 33333067, PMCID: PMC8259461, DOI: 10.1016/j.ydbio.2020.12.005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBody PatterningEmbryo, MammalianEmbryonic DevelopmentHumansMiceMorphogenesisSignal TransductionStem CellsConceptsSignaling gradientsMorphogen signaling gradientsAnteroposterior axis specificationMammalian embryosBody planAxis specificationSymmetry breakingModel of embryogenesisEmbryonic symmetryEmbryonic cellsPatterns in vivoMolecular processesAdult organismBiochemical mechanismsDynamics of morphogenesisEmbryogenesisIn vitro modelEmbryosModel systemIn vitroCritical processCellsMorphogenMorphogenesisStem cellsBMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo
Sozen B, Demir N, Zernicka-Goetz M. BMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo. Developmental Biology 2020, 470: 84-94. PMID: 33217407, PMCID: PMC8219371, DOI: 10.1016/j.ydbio.2020.11.005.Peer-Reviewed Original ResearchConceptsBMP signalingEctoderm developmentNon-canonical mechanismMouse embryosMechanism of BMPMutant embryosGenetic studiesEarly embryogenesisSignaling pathwayPost-implantation developmentBMP activityTissue organisationStem cell populationStage in vitroSpatiotemporal controlUterine tissueEmbryosCell populationsBMPStem cellsSignalImplantationPost-implantationMiceMorphogenesis