2021
Unfolded protein response triggers differential apoptotic mechanisms in ovaries and early embryos exposed to maternal type 1 diabetes
Okan A, Demir N, Sozen B. Unfolded protein response triggers differential apoptotic mechanisms in ovaries and early embryos exposed to maternal type 1 diabetes. Scientific Reports 2021, 11: 12759. PMID: 34140543, PMCID: PMC8211688, DOI: 10.1038/s41598-021-92093-3.Peer-Reviewed Original ResearchConceptsUnfolded protein responseProtein responseER stressDiabetes mellitusFemale mammalian reproductive systemEndoplasmic reticulum (ER)-stressMaternal type 1 diabetesEmbryonic developmentActivation of ER stressMammalian ovarian functionType 1 DMIn vivo mouse modelInduced developmental programAdult ovarian tissueEffect of DMMammalian reproductive systemEarly embryonic developmentEarly embryo developmentPre-implantation embryosType 1 diabetesApoptotic cascadeDiabetic ovariesMaternal DMActivated CHOPCaspase 12
2019
FoxO transcription factors 1 regulate mouse preimplantation embryo development
Kuscu N, Gungor-Ordueri N, Sozen B, Adiguzel D, Celik-Ozenci C. FoxO transcription factors 1 regulate mouse preimplantation embryo development. Journal Of Assisted Reproduction And Genetics 2019, 36: 2121-2133. PMID: 31396850, PMCID: PMC6823425, DOI: 10.1007/s10815-019-01555-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBlastocystCell Cycle CheckpointsCell Cycle ProteinsEmbryonic DevelopmentFas Ligand ProteinFemaleForkhead Box Protein O1Forkhead Box Protein O3Forkhead Transcription FactorsGene Expression Regulation, DevelopmentalHumansMiceP21-Activated KinasesPregnancySirtuin 1Tumor Suppressor Protein p53ConceptsPreimplantation embryo developmentFOXO transcription factorsFas ligandTranscription factorsRelated proteinsProteins regulating apoptosisPreimplantation embryosCell cycle related proteinsCleaved caspase 3 proteinsCell cycle arrestDevelopmental competence of embryosEmbryo developmentMouse preimplantation embryo developmentApoptosis-related proteinsMouse embryosCaspase 3 proteinTwo-cell mouse embryosResveratrol treatmentCompetence of embryosHuman preimplantation embryosMouse preimplantation embryosFOXO genesDUTP nick end labeling assayNick end labeling assayKnockdown embryos
2015
The p38 MAPK signalling pathway is required for glucose metabolism, lineage specification and embryo survival during mouse preimplantation development
Sozen B, Ozturk S, Yaba A, Demir N. The p38 MAPK signalling pathway is required for glucose metabolism, lineage specification and embryo survival during mouse preimplantation development. Cells And Development 2015, 138: 375-398. PMID: 26025760, DOI: 10.1016/j.mod.2015.05.002.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBlastocystBlastocyst Inner Cell MassCell DeathCell LineageEmbryonic DevelopmentFemaleGene Expression Regulation, DevelopmentalGlucoseGlucose Transporter Type 1Glucose Transporter Type 4ImidazolesMaleMAP Kinase Signaling SystemMiceMice, Inbred BALB CModels, BiologicalPregnancyProtein Kinase InhibitorsPyridinesRNA, MessengerConceptsPreimplantation developmentP38 mitogen-activated protein kinaseMitogen-activated protein kinaseLate preimplantation stagePreimplantation embryo developmentMouse preimplantation developmentExpression levelsEmbryo developmentEffect of p38 MAPK inhibitionExpression levels of NANOGEarly embryo developmentP38 MAPK inhibitionPresence of SB203580P38 MAPK signaling pathwayLevel of transcriptionIncreased cell deathSignal transduction pathwaysBlastocyst stageExpression levels of transcriptsLevels of NanogP38 MAPK pathwayPreimplantation stagesLevels of GLUT1Expression levels of Glut1MAPK signaling pathway
2014
Unfolded protein response prevents blastocyst formation during preimplantation embryo development in vitro
Basar M, Bozkurt I, Guzeloglu-Kayisli O, Sozen B, Tekmen I, Schatz F, Arici A, Lockwood CJ, Kayisli UA. Unfolded protein response prevents blastocyst formation during preimplantation embryo development in vitro. Fertility And Sterility 2014, 102: 1777-1784. PMID: 25305729, DOI: 10.1016/j.fertnstert.2014.09.004.Peer-Reviewed Original ResearchConceptsUnfolded protein responseProtein responseBlastocyst formationEmbryo developmentActivation of UPRPreimplantation embryo developmentTM treatmentBlastocyst developmentEffect of tunicamycinEndoplasmic reticulum stressEmbryonic developmentER stressCell embryosNuclear fragmentationMorphological differencesImmunoglobulin proteinBlastocyst stageReticulum stressTunicamycinDevelopment ratePregnant mare serum gonadotropinBlastocyst development rateProtein-1 mRNA expressionNongenomic mechanismsEmbryos
2012
Merit of quinacrine in the decrease of ingested sulfite-induced toxic action in rat brain
Kencebay C, Derin N, Ozsoy O, Kipmen-Korgun D, Tanriover G, Ozturk N, Basaranlar G, Yargicoglu-Akkiraz P, Sozen B, Agar A. Merit of quinacrine in the decrease of ingested sulfite-induced toxic action in rat brain. Food And Chemical Toxicology 2012, 52: 129-136. PMID: 23168241, DOI: 10.1016/j.fct.2012.11.015.Peer-Reviewed Original ResearchConceptsSomatosensory evoked potentialsSecretory phospholipase A2Quinacrine administrationS groupPlasma-S-sulfonate levelLatencies of SEP componentsMale albino Wistar ratsBrain TBARS levelsControl levelsTUNEL-positive cellsSQ groupTBARS levelsAlbino Wistar ratsSPLA2 levelsApoptotic cell deathGastric gavageSEP alterationsPositive cellsWistar ratsSEP componentsIntraperitoneal injectionRat brainCaspase-3RatsQuinacrine treatment