2017
Hepatitis C Virus Indirectly Disrupts DNA Damage-Induced p53 Responses by Activating Protein Kinase R
Mitchell JK, Midkiff BR, Israelow B, Evans MJ, Lanford RE, Walker CM, Lemon SM, McGivern DR. Hepatitis C Virus Indirectly Disrupts DNA Damage-Induced p53 Responses by Activating Protein Kinase R. MBio 2017, 8: e00121-17. PMID: 28442604, PMCID: PMC5405228, DOI: 10.1128/mbio.00121-17.Peer-Reviewed Original ResearchConceptsHepatitis C virusHCV infectionC virusProtein kinase RHCV replicationLiver cancerHost responseHepatocellular carcinogenesisP53 functionHCV protein expressionHCV RNA replicationP53 responseLiver-derived HepG2 cellsDNA damageOncogenic RNA virusesInfectious causesChronic infectionRNA replicationPhosphorylated protein kinase RActivation of p53Viral activationInfectionMicroRNA-122P53-mediated responseP53 inhibition
2014
Hepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors
Israelow B, Mullokandov G, Agudo J, Sourisseau M, Bashir A, Maldonado AY, Dar AC, Brown BD, Evans MJ. Hepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors. Nature Communications 2014, 5: 5408. PMID: 25403145, PMCID: PMC4236719, DOI: 10.1038/ncomms6408.Peer-Reviewed Original ResearchConceptsMiR-122Hepatitis C virus replicationRecent clinical trialsC virus replicationMiR-122 inhibitionMiR-122 inhibitorClinical trialsHCV replicationMiR-122 knockdownHCV drugsHCV variantsHCVHCV genomeViral titresVirus replicationNext-generation sequencingTransient inhibitionVirus geneticsViral adaptationViral sequencesInhibitionNucleotide changesSingle nucleotide changePatientsTitresHepG2 cells mount an effective antiviral interferon‐lambda based innate immune response to hepatitis C virus infection
Israelow B, Narbus CM, Sourisseau M, Evans MJ. HepG2 cells mount an effective antiviral interferon‐lambda based innate immune response to hepatitis C virus infection. Hepatology 2014, 60: 1170-1179. PMID: 24833036, PMCID: PMC4176518, DOI: 10.1002/hep.27227.Peer-Reviewed Original ResearchConceptsInnate immune responseHCV infectionHuh-7.5 cellsInnate immune systemImmune responseImmune systemAntiviral responseEntire HCV life cycleHepatitis C virus exposurePersistent life-long infectionHepatitis C virus infectionMelanoma differentiation-associated protein 5C virus infectionRobust antiviral responseImportance of IFNHuh-7Retinoic acid-inducible gene IHCV life cycleAcid-inducible gene ILife-long infectionInnate immune antagonistsHepG2 cellsLike receptor pathwayLiver-specific microRNAHCV recognition