Detection of the Mutated K-Ras Biomarker in Colorectal Carcinoma
Doolittle B, Emanuel J, Tuttle C, Costa J. Detection of the Mutated K-Ras Biomarker in Colorectal Carcinoma. Experimental And Molecular Pathology 2001, 70: 289-301. PMID: 11418008, DOI: 10.1006/exmp.2001.2364.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkers, TumorColorectal NeoplasmsDeoxyribonuclease EcoRIDNADNA, NeoplasmFecesGenes, rasHumansIndicators and ReagentsMutationPolymerase Chain ReactionPolymorphism, Single-Stranded ConformationalQuaternary Ammonium CompoundsReference ValuesReproducibility of ResultsSite-Specific DNA-Methyltransferase (Adenine-Specific)Trimethyl Ammonium CompoundsConceptsColorectal carcinomaStool samplesHealthy patientsK-rasReliable screening testDouble-blind formatK-ras mutationsWild-type K-rasK-ras oncogeneK-ras sequencesCommon malignancyCRC tumorsPolymerase chain reactionEarly lesionsStool DNANoninvasive screenScreening testTumor tissueGenetic biomarkersHuman stoolPatientsEarly detectionHigh false positive rateSingle-strand conformation polymorphismTissue DNA