2013
Decision analysis model of open repair versus endovascular treatment in patients with asymptomatic popliteal artery aneurysms
Hogendoorn W, Schlösser FJ, Moll FL, Muhs BE, Hunink MG, Sumpio BE. Decision analysis model of open repair versus endovascular treatment in patients with asymptomatic popliteal artery aneurysms. Journal Of Vascular Surgery 2013, 59: 651-662.e2. PMID: 24246533, DOI: 10.1016/j.jvs.2013.09.026.Peer-Reviewed Original ResearchMeSH KeywordsAgedAged, 80 and overAneurysmAnimalsAsymptomatic DiseasesCardiovascular AgentsCatsComputer SimulationCost-Benefit AnalysisDecision Support TechniquesEndovascular ProceduresHospital CostsHumansMaleMarkov ChainsMonte Carlo MethodPatient SelectionPopliteal ArteryQuality of LifeQuality-Adjusted Life YearsRisk FactorsSaphenous VeinStentsTreatment OutcomeVascular Patency
2011
Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation
Abe R, Beckett J, Abe R, Nixon A, Rochier A, Yamashita N, Sumpio B. Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation. European Journal Of Vascular And Endovascular Surgery 2011, 41: 814-820. PMID: 21333557, DOI: 10.1016/j.ejvs.2010.12.021.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationCell cycle analysisSMC proliferationCell proliferationInhibits Smooth Muscle Cell ProliferationVascular smooth muscle cell proliferationCoronary artery diseaseNon-treated groupExtracellular signal-regulated kinase 1/2 activationCardiovascular mortalityArtery diseaseKinase 1/2 activationCell cycle blockG1-S phaseMediterranean dietDay 1Oleuropein treatmentGrowth of SMCsG1 phase regulatorsFlow cytometryVascular SMCsPresence of oleuropeinBeneficial effectsCycle analysis
2010
Thrombospondin 1, Fibronectin, and Vitronectin are Differentially Dependent Upon RAS, ERK1/2, and p38 for Induction of Vascular Smooth Muscle Cell Chemotaxis
Willis AI, Sadowitz B, Fuse S, Maier KG, Lee TS, Wang XJ, Tuszynski GP, Sumpio BE, Gahtan V. Thrombospondin 1, Fibronectin, and Vitronectin are Differentially Dependent Upon RAS, ERK1/2, and p38 for Induction of Vascular Smooth Muscle Cell Chemotaxis. Vascular And Endovascular Surgery 2010, 45: 55-62. PMID: 21193465, DOI: 10.1177/1538574410387677.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsCattleCells, CulturedChemotaxisFibronectinsHumansMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Muscle, Smooth, VascularMyocytes, Smooth MuscleP38 Mitogen-Activated Protein KinasesProtein Kinase InhibitorsRas ProteinsThrombospondin 1TransfectionVitronectinConceptsVascular smooth muscle cellsThrombospondin-1Smooth muscle cell chemotaxisFarnesyl protein transferase inhibitorSignal transduction pathwaysProtein transferase inhibitorsVascular smooth muscle cell chemotaxisBovine vascular smooth muscle cellsTSP-1Ras N17Transduction pathwaysSecond messenger systemsP38Smooth muscle cellsERK1/2VSMC migrationTransferase inhibitorsPD098059Cell chemotaxisSB202190Muscle cellsMessenger systemsVitronectinRAChemotaxisThe Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty
Basco MT, Yiu WK, Cheng SW, Sumpio BE. The Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty. Journal Of Vascular And Interventional Radiology 2010, 21: 910-915. PMID: 20417120, PMCID: PMC2878641, DOI: 10.1016/j.jvir.2010.02.016.Peer-Reviewed Original Research
2009
Lovastatin Inhibits Thrombospondin-1-Induced Smooth Muscle Cell Chemotaxis1
Esemuede N, Lee T, Maier KG, Sumpio BE, Gahtan V. Lovastatin Inhibits Thrombospondin-1-Induced Smooth Muscle Cell Chemotaxis1. Journal Of Surgical Research 2009, 168: 149-154. PMID: 20338582, DOI: 10.1016/j.jss.2009.11.728.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell ProliferationCells, CulturedChemotaxisDose-Response Relationship, DrugHydroxymethylglutaryl-CoA Reductase InhibitorsLovastatinMevalonic AcidModels, AnimalMonomeric GTP-Binding ProteinsMuscle, Smooth, VascularRas ProteinsSignal TransductionThrombospondin 1Tunica IntimaTunica MediaConceptsTSP-1-induced chemotaxisGeranylgeranyl transferase inhibitorSerum-free mediumThrombospondin-1HMG-CoA reductase inhibitorsVascular smooth muscle cell migrationSmooth muscle cell migrationTransferase inhibitorsEffect of lovastatinG proteinsMuscle cell migrationRas activationRho-kinase inhibitorCell migrationPost-hoc testingFarnesyl transferase inhibitorsCholesterol loweringLovastatin doseVascular restenosisIntimal hyperplasiaBoyden chamberPleiotropic propertiesReductase inhibitorsWestern blotInhibition of Ras
2008
The role of G proteins in thromospondin-1–induced vascular smooth muscle cell migration
Fuse S, Esemuede N, Yamaguchi M, Maier KG, Nesselroth SM, Sumpio BE, Gahtan V. The role of G proteins in thromospondin-1–induced vascular smooth muscle cell migration. Surgery 2008, 144: 86-92. PMID: 18571589, DOI: 10.1016/j.surg.2008.03.028.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsTSP-1-induced chemotaxisExtracellular signal-regulated kinase 1/2Pertussis toxinSerum-free mediumCholera toxinThrombospondin-1CAMP levelsP38 activationG proteinsVascular smooth muscle cell migrationQuiescent vascular smooth muscle cellsSmooth muscle cell migrationCyclic adenosine monophosphate levelsSmooth muscle cellsIntracellular cyclic adenosine monophosphate (cAMP) levelsMuscle cell migrationTSP-1 receptorAdenosine monophosphate levelsSignal-regulated kinase 1/2Levels of cAMPArterial lesionsVascular injuryVSMC chemotaxisMicrochemotaxis chamberSynergistic Effect of Cool/Thaw Cycles on Vascular Cells in an In Vitro Model of Cryoplasty
Yiu WK, Cheng SW, Sumpio BE. Synergistic Effect of Cool/Thaw Cycles on Vascular Cells in an In Vitro Model of Cryoplasty. Journal Of Vascular And Interventional Radiology 2008, 19: 925-930. PMID: 18503909, DOI: 10.1016/j.jvir.2008.02.007.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsAkt activationEndothelial cellsApoptotic smooth muscle cellsActivation of AktBovine aortic smooth muscle cellsAortic smooth muscle cellsSurvival responseDeoxynucleotidyl transferase-mediated dUTP nick end labelingFetal bovine serumTerminal deoxynucleotidyl transferase-mediated dUTP nick end labelingTransferase-mediated dUTP nick end labelingImmunoblot analysisDUTP nick end labelingHigher apoptotic rateVascular cellsThrombospondin-1-Induced Migration Is Functionally Dependent Upon Focal Adhesion Kinase
Wang XJ, Maier K, Fuse S, Willis AI, Olson E, Nesselroth S, Sumpio BE, Gahtan V. Thrombospondin-1-Induced Migration Is Functionally Dependent Upon Focal Adhesion Kinase. Vascular And Endovascular Surgery 2008, 42: 256-262. PMID: 18319354, DOI: 10.1177/1538574408314440.Peer-Reviewed Original ResearchDifferential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells
Kim JI, Cordova AC, Hirayama Y, Madri JA, Sumpio BE. Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells. Endothelium 2008, 15: 33-42. PMID: 18568943, PMCID: PMC2644408, DOI: 10.1080/10623320802092260.Peer-Reviewed Original ResearchConceptsSp1 phosphorylationMT1-MMP expressionPromoter sitesPKCzeta inhibitorProtein kinase CzetaAffinity of Sp1Egr-1 bindingProtein kinase CζExtracellular matrix remodelingEndothelial cell migrationSp1Cell migrationPhosphorylationMatrix remodelingProtein expressionCyclic strainExpressionMembrane typeEndothelial cellsKey roleCzetaInhibitorsCζMetalloproteinaseAffinity
2007
Utility of the aortic fenestration technique in the management of acute aortic dissections.
Pradhan S, Elefteriades JA, Sumpio BE. Utility of the aortic fenestration technique in the management of acute aortic dissections. Annals Of Thoracic And Cardiovascular Surgery 2007, 13: 296-300. PMID: 17954985.Peer-Reviewed Original ResearchConceptsAortic replacement surgeryPercutaneous balloon fenestrationSurgical aortic fenestrationAcute aortic dissectionAortic fenestrationAortic dissectionBalloon fenestrationReplacement surgeryType B acute aortic dissectionB acute aortic dissectionLong-term effectivenessFenestration techniqueVessel ischemiaMedical managementHigh morbidityOptimal treatmentOpen fenestrationInvasive approachOperative strategySafe alternativeFenestrationPatientsSurgeryRecent dataEffective alternativeDirect comparison of endothelial cell and smooth muscle cell response to supercooling and rewarming
Yiu WK, Cheng SW, Sumpio BE. Direct comparison of endothelial cell and smooth muscle cell response to supercooling and rewarming. Journal Of Vascular Surgery 2007, 46: 557-564.e2. PMID: 17826245, DOI: 10.1016/j.jvs.2007.04.072.Peer-Reviewed Original ResearchAnimalsAorta, ThoracicApoptosisBlotting, WesternCattleCell ProliferationCells, CulturedCryopreservationDensitometryEndothelial CellsEnzyme ActivationIn Situ Nick-End LabelingMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Muscle, Smooth, VascularPhosphorylationProto-Oncogene Proteins c-aktRewarmingEffects of different types of fluid shear stress on endothelial cell proliferation and survival
Kadohama T, Nishimura K, Hoshino Y, Sasajima T, Sumpio BE. Effects of different types of fluid shear stress on endothelial cell proliferation and survival. Journal Of Cellular Physiology 2007, 212: 244-251. PMID: 17323381, DOI: 10.1002/jcp.21024.Peer-Reviewed Original ResearchPhosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain
Hoshino Y, Nishimura K, Sumpio BE. Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain. Journal Of Cellular Biochemistry 2007, 100: 515-526. PMID: 16927376, DOI: 10.1002/jcb.21085.Peer-Reviewed Original ResearchConceptsCasein kinase 2Transfection of ECsPTEN plasmidVascular cell morphologyEndothelial cellsPI3K activitySuppression of apoptosisPI3K-Akt pathwayLipid phosphataseMaximal activityPhosphatase PTENPhospho-PTENPTEN activityBovine aortic endothelial cellsIntracellular phosphatidylinositolUpstream regulatorAkt activityKinase 2Phospho-AKT activityK activityTime-dependent mannerAortic endothelial cellsCell morphologyCell proliferationPTENRole of Ligand-Specific Integrins in Endothelial Cell Alignment and Elongation Induced by Cyclic Strain
Hirayama Y, Sumpio BE. Role of Ligand-Specific Integrins in Endothelial Cell Alignment and Elongation Induced by Cyclic Strain. Endothelium 2007, 14: 275-283. PMID: 18080865, DOI: 10.1080/10623320701746248.Peer-Reviewed Original ResearchConceptsEndothelial cellsP38 MAPKAnti-alpha2 integrin antibodyMorphological changesP38 mitogen-activated protein kinase (MAPK) pathwayCycles/minIntegrin-blocking antibodiesP38 MAPK pathwayMitogen-activated protein kinase pathwayAlpha 5Integrin antibodyProtein kinase pathwayMAPK pathwayAntibodiesKinase pathwayMAPKMultiple biological processesActivationIntegrinsCritical roleCyclic strainPathway
2006
Vascular Smooth Muscle Cell Apoptosis Induced by “Supercooling” and Rewarming
Yiu WK, Cheng SW, Sumpio BE. Vascular Smooth Muscle Cell Apoptosis Induced by “Supercooling” and Rewarming. Journal Of Vascular And Interventional Radiology 2006, 17: 1971-1977. PMID: 17185696, DOI: 10.1097/01.rvi.0000244868.65867.fb.Peer-Reviewed Original ResearchPECAM-1 Affects GSK-3β-Mediated β-Catenin Phosphorylation and Degradation
Biswas P, Canosa S, Schoenfeld D, Schoenfeld J, Li P, Cheas LC, Zhang J, Cordova A, Sumpio B, Madri JA. PECAM-1 Affects GSK-3β-Mediated β-Catenin Phosphorylation and Degradation. American Journal Of Pathology 2006, 169: 314-324. PMID: 16816383, PMCID: PMC1698776, DOI: 10.2353/ajpath.2006.051112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta CateninBlotting, WesternCapillary PermeabilityCells, CulturedEndothelial CellsFluorescent Antibody TechniqueGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHistamineHistamine AgentsHumansMiceModels, BiologicalPhosphatidylinositol 3-KinasesPhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Proto-Oncogene Proteins c-aktReceptors, HistamineSignal TransductionConceptsAdherens junctionsSerine phosphorylationSrc homology 2 domainBeta-catenin expression levelsAdherens junction componentsSerine phosphorylation levelEndothelial cellsΒ-catenin phosphorylationPECAM-1Cell biological responsesCytoplasmic domainSHP-2Proteosomal degradationGSK-3betaDynamic regulatorJunction componentsPhosphorylation levelsPhosphorylationEndothelial cell adhesion molecule-1Expression levelsGSK-3βBiological responsesEndothelial barrier permeabilityMice exhibitCell adhesion molecule-1Role of AKT in cyclic strain-induced endothelial cell proliferation and survival
Nishimura K, Li W, Hoshino Y, Kadohama T, Asada H, Ohgi S, Sumpio BE. Role of AKT in cyclic strain-induced endothelial cell proliferation and survival. American Journal Of Physiology - Cell Physiology 2006, 290: c812-c821. PMID: 16469863, DOI: 10.1152/ajpcell.00347.2005.Peer-Reviewed Original ResearchConceptsEndothelial cellsCycles/minRole of AktEC proliferationCultured bovine aortic endothelial cellsSurvival of ECsAortic endothelial cellsApoptotic endothelial cellsGSK-3betaBovine aortic endothelial cellsEndothelial cell proliferationFrequency of strainsPositive cellsGlycogen synthase kinaseKinase-dead AktCell proliferationCaspase-3AktSurvivalCell numberSynthase kinaseProliferationEarly phosphorylationBad phosphorylationp38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress
Kadohama T, Akasaka N, Nishimura K, Hoshino Y, Sasajima T, Sumpio BE. p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress. Endothelium 2006, 13: 43-50. PMID: 16885066, DOI: 10.1080/10623320600660219.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAtherosclerosisBlood PressureBlood VesselsCattleCell PolarityCell ShapeCells, CulturedDose-Response Relationship, DrugEndothelial CellsEnzyme ActivationEnzyme InhibitorsHemodynamicsMAP Kinase Signaling SystemP38 Mitogen-Activated Protein KinasesPhosphorylationRegional Blood FlowStress, MechanicalTime FactorsConceptsStatic endothelial cellsFluid shear stressP38 MAPKP38 Mitogen-Activated Protein Kinase ActivationMitogen-Activated Protein Kinase ActivationEndothelial cellsProtein kinase activationP38 MAPK inhibitor SB-203580Inhibitor SB 203580Cell alignmentProtein kinaseBovine aortic endothelial cellsLaminar shear stressKinase activationSB 203580P38 mitogenCultured bovine aortic endothelial cellsEC elongationAortic endothelial cellsMAPKCellsElongationActivationMorphometric analysisKinase
2005
Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis
Poussier B, Cordova AC, Becquemin JP, Sumpio BE. Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis. Journal Of Vascular Surgery 2005, 42: 1190-1190.e14. PMID: 16376213, DOI: 10.1016/j.jvs.2005.08.014.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis InhibitorsAnimalsAnimals, NewbornAntioxidantsAorta, ThoracicApoptosisBlotting, WesternCattleCell ProliferationCells, CulturedDNADose-Response Relationship, DrugFlow CytometryIn Situ Nick-End LabelingIn Vitro TechniquesMuscle, Smooth, VascularProliferating Cell Nuclear AntigenResveratrolStilbenesConceptsSmooth muscle cell proliferationMuscle cell proliferationVascular smooth muscle cell proliferationDose-dependent mannerSMC proliferationCell proliferationG1-S phaseBeneficial effectsFrench paradoxDose-dependent apoptotic effectTerminal deoxynucleotidyl transferase-mediated dUTP-biotin nickTransferase-mediated dUTP-biotin nickCardiovascular death ratesRed wine intakeRed wine consumptionEffects of resveratrolAortic SMC proliferationEnd labeling stainingVascular SMC proliferationDUTP-biotin nickMajor polyphenol componentHealthy life styleCell cycle analysisCardiovascular mortalityFlow-activated cell sortingDifferential responsiveness of early- and late-passage endothelial cells to shear stress
Kudo FA, Warycha B, Juran PJ, Asada H, Teso D, Aziz F, Frattini J, Sumpio BE, Nishibe T, Cha C, Dardik A. Differential responsiveness of early- and late-passage endothelial cells to shear stress. The American Journal Of Surgery 2005, 190: 763-769. PMID: 16226955, DOI: 10.1016/j.amjsurg.2005.07.017.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAortaApoptosisBlotting, WesternCattleCell CountCell DivisionCell ProliferationCells, CulturedEndothelium, VascularIn Vitro TechniquesMuscle, Smooth, VascularPhosphorylationProliferating Cell Nuclear AntigenProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthStress, MechanicalTumor Suppressor Protein p53ConceptsLate passage endothelial cellsOrbital shear stressEarly passage cellsSmooth muscle cell migrationMuscle cell migrationEndothelial cellsSenescence modelAkt phosphorylationCell migrationProtein kinase B activationPassage cellsKinase B activationCell proliferationVascular disease increasesLate passage cellsBovine aortic endothelial cellsNuclear antigen reactivityAortic endothelial cellsEndothelial cell proliferationNeointimal hyperplasiaAntigen reactivityTotal AktBoyden chamberB activationWestern blotting