2002
Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration
Throckmorton D, Kurscheid-Reich D, Rosales OR, Rodriguez-Commes J, Lopez R, Sumpio B, Zhong Q, Ding K, McCarthy R, Barrett PQ, Isales CM. Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration. Peptides 2002, 23: 79-85. PMID: 11814621, DOI: 10.1016/s0196-9781(01)00582-4.Peer-Reviewed Original ResearchConceptsParathyroid hormoneEndothelial cell lineVoltage-sensitive calcium channelsParathyroid hormone effectsSensitive calcium channelsUmbilical vein endothelial cell lineHuman umbilical vein endothelial cell lineCell linesProtein kinase CCalcium channelsHormone effectsEndothelial cellsFurther studiesPhospholipid turnoverPeptide concentrationPKC translocationSpecific effectsKinase CCell homogenatesPKC isoformsParticulate fraction
2000
Functional parathyroid hormone receptors are present in an umbilical vein endothelial cell line
Isales C, Sumpio B, Bollag R, Zhong Q, Ding K, Du W, Rodriguez-Commes J, Lopez R, Rosales O, Gasalla-Herraiz J, McCarthy R, Barrett P. Functional parathyroid hormone receptors are present in an umbilical vein endothelial cell line. AJP Endocrinology And Metabolism 2000, 279: e654-e662. PMID: 10950835, DOI: 10.1152/ajpendo.2000.279.3.e654.Peer-Reviewed Original ResearchMeSH KeywordsCalciumCell LineChromatography, High Pressure LiquidCyclic AMPEndothelin-1Endothelium, VascularFluorescent DyesFura-2Glyceraldehyde-3-Phosphate DehydrogenasesHumansInositol PhosphatesParathyroid HormoneReceptors, Parathyroid HormoneReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSignal TransductionThymidineUmbilical VeinsConceptsParathyroid hormoneUmbilical vein endothelial cell lineParathyroid hormone receptorEndothelial cell lineHormone receptorsVascular smooth muscle relaxationEndothelin-1 secretionSmooth muscle relaxationVascular smooth muscleHuman umbilical vein endothelial cell lineCell linesCellular cAMP contentPossible direct effectPeptide receptor mRNAEndothelium-derived cellsBlood pressureContinuous infusionMuscle relaxationIntracellular calciumSmooth muscleReceptor mRNAHormone exposureVascular endotheliumEndothelial cellsCAMP content
1999
Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain
Ikeda M, Takei T, Mills I, Kito H, Sumpio B. Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain. American Journal Of Physiology 1999, 276: h614-h622. PMID: 9950863, DOI: 10.1152/ajpheart.1999.276.2.h614.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCalciumCalcium-Calmodulin-Dependent Protein KinasesCattleCell DivisionCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsExtracellular SpaceFlavonoidsIntracellular MembranesPhosphorylationProtein Kinase CProtein-Tyrosine KinasesStress, MechanicalConceptsERK1/ERK2Bovine aortic endothelial cellsExtracellular signal-regulated kinases 1ERK1/ERK2 activationExtracellular signal-regulated kinases 1/2Signal-regulated kinases 1/2Tyrosine kinase inhibitor genisteinCell proliferationTreatment of BAECProtein kinase C activationProtein kinase C inhibitor calphostin CTime-dependent phosphorylationKinase inhibitor genisteinKinase C activationERK2 activationEndothelial cellsERK2Strain-induced activationKinase 1Kinase 1/2PD 98059Tyrosine kinaseInhibitor genisteinCalphostin CStrain-dependent activation
1998
Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain
Ikeda M, Takei T, Mills I, Sumpio B. Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain. Biochemical And Biophysical Research Communications 1998, 247: 462-465. PMID: 9642151, DOI: 10.1006/bbrc.1998.8811.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channel BlockersCalcium-Calmodulin-Dependent Protein KinasesCattleCells, CulturedChelating AgentsEgtazic AcidEndothelium, VascularEnzyme ActivationExtracellular SpaceGadoliniumHydroquinonesIntracellular FluidMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationStress, MechanicalConceptsEndothelial cellsActivation of ERK1/2Baseline phosphorylationExtracellular Ca2Normal extracellular Ca2Aortic endothelial cellsKinase 1Bovine aortic endothelial cellsStrain-induced activationCycles/minChannel blockersExtracellular signal-regulated kinases 1Signal-regulated kinases 1Calcium-independent activationBenzohydroquinoneERK1/2 activationERK1/2Free mediumEffect of Ca2ActivationCa2EGTA
1997
Exposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools
ROSALES O, ISALES C, BARRETT P, BROPHY C, SUMPIO B. Exposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools. Biochemical Journal 1997, 326: 385-392. PMID: 9291109, PMCID: PMC1218682, DOI: 10.1042/bj3260385.Peer-Reviewed Original ResearchConceptsMicroM GdCl3Endothelial cellsIP3 formationLate-phase componentsInitiation of stretchExtracellular poolConfluent bovine aortic endothelial cellsMobilization of intracellularAortic endothelial cellsBovine aortic endothelial cellsNM charybdotoxinCycles/minIP3 generationIntracellular Ca2Sustained phaseCytosolic Ca2Confluent endothelial cellsBenzohydroquinoneFree mediumTemporal relationshipMicroM NiCl2Ca2PreincubationCellsInitial rise
1995
Endothelin-1 increases intracellular calcium in human monocytes and causes production of interleukin-6
McMillen M, Huribal M, Cunningham M, Kumar R, Sumpio B. Endothelin-1 increases intracellular calcium in human monocytes and causes production of interleukin-6. Critical Care Medicine 1995, 23: 34-40.. PMID: 8001384, DOI: 10.1097/00003246-199501000-00009.Peer-Reviewed Original ResearchConceptsEndothelin-1Intracellular calciumHuman monocytesIL-6Production of interleukinIL-6 productionIL-1 productionIntracellular calcium concentrationBasic science laboratoryM endothelinInterleukin-6Healthy donorsFura-2Control monocytesIschemic endotheliumMonocytesEndothelinCalcium concentrationMean valueCalciumTissue cultureInterleukinPhytohemagglutininEndotheliumEndotoxin
1987
Mitochondrial Respiratory and Calcium Studies
SUMPIO B, BAUE A, CHAUDRY I. Mitochondrial Respiratory and Calcium Studies. Annals Of Surgery 1987, 206: 655-660. PMID: 3499878, PMCID: PMC1493310, DOI: 10.1097/00000658-198711000-00017.Peer-Reviewed Original ResearchConceptsRespiratory control ratioVerapamil pretreatmentTotal tissue Ca2Tissue ATP levelsCyA-induced nephrotoxicityCyA treatmentPharmacologic regimenRenal functionMitochondrial respiratory functionSimultaneous administrationCalcium studiesRespiratory functionKrebs bufferCortical mitochondriaMumol/Tissue Ca2Rat kidneyADP/O ratioCyANephrotoxicityATP levelsControl ratioVerapamilMitochondrial respiratoryCa2