2011
Varying Effects of Hemodynamic Forces on Tissue Factor RNA Expression in Human Endothelial Cells
Abe R, Yamashita N, Rochier A, Nixon A, Abe R, Madri JA, Sumpio BE. Varying Effects of Hemodynamic Forces on Tissue Factor RNA Expression in Human Endothelial Cells. Journal Of Surgical Research 2011, 170: 150-156. PMID: 21592524, DOI: 10.1016/j.jss.2011.04.002.Peer-Reviewed Original ResearchConceptsLaminar flowOscillatory flowCyclic strainUnidirectional laminar flowUniform laminar flowMechanical stressDisturbed flowTF RNA expressionHuman umbilical vein endothelial cellsFlowStatic controlHigh TF expressionTF expressionForceMechanical forcesEndothelial cellsRNA expressionSustained amplification
2002
Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*
Yun S, Dardik A, Haga M, Yamashita A, Yamaguchi S, Koh Y, Madri JA, Sumpio BE. Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*. Journal Of Biological Chemistry 2002, 277: 34808-34814. PMID: 12093818, DOI: 10.1074/jbc.m205417200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCells, CulturedDNADNA-Binding ProteinsEarly Growth Response Protein 1Electrophoretic Mobility Shift AssayEndothelium, VascularImmediate-Early ProteinsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNogalamycinPhosphorylationPromoter Regions, GeneticRatsRats, Sprague-DawleyRNA, MessengerSp1 Transcription FactorStress, PhysiologicalTranscription FactorsConceptsMT1-MMP expressionEgr-1MRNA transcriptionMT1-MMP promoterPost-translational modificationsCalf intestinal phosphataseDistinct environmental stimuliTranscription factor expressionSp1 phosphorylationEgr-1 expressionSp1 DNAEndothelial cell migrationSerine phosphorylationPromoter sitesSp1Cell migrationEnvironmental stimuliMatrix remodelingIntestinal phosphataseProtein levelsTranscriptionTime-dependent fashionPhosphorylationMechanical forcesExpression
1997
Gene Regulation by Mechanical Forces
Oluwole BO, Du W, Mills I, Sumpio BE. Gene Regulation by Mechanical Forces. Endothelium 1997, 5: 85-93. PMID: 9237042, DOI: 10.3109/10623329709079866.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell Adhesion MoleculesCell Culture TechniquesCells, CulturedEndothelinsEndothelium, VascularEpoprostenolFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression RegulationHemorheologyHumansMechanoreceptorsMembranes, ArtificialNitric OxideProtein-Tyrosine KinasesSignal TransductionStress, MechanicalTissue Plasminogen ActivatorTranscription FactorsTranscription, GeneticVacuumConceptsEndothelial cellsEndothelial cell functionCyclic strainVasoactive mediatorsSecond messenger cascadesNitric oxideBlood vesselsMechanical forcesCell functionMechanical deformationFlow of bloodMessenger cascadesLuminal surfaceSpecific response elementsPromoter geneResponse elementExternal forcesMediatorsCytoskeletal changesCellsForceProstacyclinExpressionTranscription levels
1994
Signal transduction pathways in vascular cells exposed to cyclic strain
Sumpio B, Du W, Cohen C, Evans L, Isales C, Rosales O, Mills I. Signal transduction pathways in vascular cells exposed to cyclic strain. 1994, 3-22. DOI: 10.1017/cbo9780511629068.001.Peer-Reviewed Original Research