2018
Regulation of Yes-Associated Protein by Laminar Flow
Chitragari G, Shalaby SY, Sumpio BJ, Kurita J, Sumpio BE. Regulation of Yes-Associated Protein by Laminar Flow. Annals Of Vascular Surgery 2018, 52: 183-191. PMID: 29758328, DOI: 10.1016/j.avsg.2018.03.002.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingCell ShapeCells, CulturedCytoskeletonDown-RegulationHuman Umbilical Vein Endothelial CellsHumansMechanotransduction, CellularPhosphoproteinsPhosphorylationProteolysisPulsatile FlowRegional Blood FlowStress, MechanicalTime FactorsTranscription FactorsYAP-Signaling ProteinsConceptsHuman umbilical vein endothelial cellsTotal YAPExposure of HUVECsRelease of mediatorsYAP levelsSignificant decreaseUbiquitin-proteasome system inhibitorsUmbilical vein endothelial cellsEndothelial cell proliferationVein endothelial cellsVascular healthSystem inhibitorsYes-Associated ProteinDisease modulationIntimal hyperplasiaDisturbed flowEndothelial cellsBlood vesselsDisease statesPotential targetCell proliferationProteasome-independent mechanismExposureKey regulatorPYAP
2016
Systemic and cell-specific mechanisms of vasculopathy induced by human immunodeficiency virus and highly active antiretroviral therapy
Haser GC, Sumpio B. Systemic and cell-specific mechanisms of vasculopathy induced by human immunodeficiency virus and highly active antiretroviral therapy. Journal Of Vascular Surgery 2016, 65: 849-859. PMID: 26994951, DOI: 10.1016/j.jvs.2016.01.036.Peer-Reviewed Original ResearchConceptsHuman immunodeficiency virusActive antiretroviral therapySmooth muscle cellsAntiretroviral therapyHIV patientsImmunodeficiency virusTraditional cardiovascular risk factorsMuscle cellsEndothelial cellsCardiovascular risk factorsChronic immune activationSerious cardiovascular eventsDevelopment of atherosclerosisVascular systemCardiovascular eventsVascular complicationsHIV medicationsChronic inflammationImmune activationMyocardial infarctionRisk factorsCell-specific mechanismsGeneral populationCholesterol metabolismCardiovascular pathology
2014
Effect of Pulsatile and Continuous Flow on Yes-Associated Protein
Chitragari G, Shalaby SY, Sumpio BJ, Sumpio BE. Effect of Pulsatile and Continuous Flow on Yes-Associated Protein. International Journal Of Angiology 2014, 23: 183-186. PMID: 25317030, PMCID: PMC4169103, DOI: 10.1055/s-0034-1376865.Peer-Reviewed Original ResearchHuman umbilical vein endothelial cellsContinuous forward flowTotal YAPPhosphorylated YAPEndothelial cellsPathogenesis of atherosclerosisEffects of pulsatileRole of YAPUmbilical vein endothelial cellsVein endothelial cellsYes-Associated ProteinMild decreaseFlow chamber systemFibronectin-coated glass slidesParallel plate flow chamber systemSignificant decreaseLonger durationSignificant differencesAtherosclerosisDifferential effectsLevel of phosphorylationForward flowMinutesYAPPFFAfrican Trypanosome-Induced Blood–Brain Barrier Dysfunction under Shear Stress May Not Require ERK Activation
Sumpio BJ, Chitragari G, Moriguchi T, Shalaby S, Pappas-Brown V, Khan AM, Sekaran SD, Sumpio BE, Grab DJ. African Trypanosome-Induced Blood–Brain Barrier Dysfunction under Shear Stress May Not Require ERK Activation. International Journal Of Angiology 2014, 24: 41-46. PMID: 27053915, PMCID: PMC4813428, DOI: 10.1055/s-0034-1370890.Peer-Reviewed Original ResearchHuman brain microvascular endothelial cellsBrain microvascular endothelial cellsTransendothelial electrical resistanceBlood-brain barrierBrain postcapillary venulesBrain barrier dysfunctionMicrovascular endothelial cellsPhosphorylation of ERK1/2Parasite cysteine proteasesNeurological symptomsBBB permeabilityBarrier dysfunctionExtracellular signal-regulated kinaseBrain capillariesMicrovascular permeabilityAbsence of trypanosomesHuman African trypanosomiasisSignal-regulated kinasePostcapillary venulesTransient increaseEndothelial cellsBarrier functionElectric cell-substrate impedance sensingProtozoan parasiteTrypanosome infectionCharacterization of Extracellular Signal-Regulated Kinase 5 Levels in Human Umbilical Vein Endothelial Cells Exposed to Disturbed and Uniform Flow
Shalaby S, Chitragari G, Sumpio BJ, Sumpio BE. Characterization of Extracellular Signal-Regulated Kinase 5 Levels in Human Umbilical Vein Endothelial Cells Exposed to Disturbed and Uniform Flow. International Journal Of Angiology 2014, 23: 187-192. PMID: 25317031, PMCID: PMC4169104, DOI: 10.1055/s-0034-1378136.Peer-Reviewed Original Research
2013
Varying effects of different types of hemodynamic forces on tissue factor (TF) expression in endothelial cells (EC)
Abe R, Yamashita N, Rochier A, Nixon A, Abe R, Moriguchi T, Sumpio B, Oda S, Hirasawa H. Varying effects of different types of hemodynamic forces on tissue factor (TF) expression in endothelial cells (EC). Cardiovascular Pathology 2013, 22: e49-e50. DOI: 10.1016/j.carpath.2013.01.071.Peer-Reviewed Original Research
2012
169
Oda S, Hirasawa H, Sumpio B. 169. Critical Care Medicine 2012, 40: 1-328. DOI: 10.1097/01.ccm.0000424389.66537.91.Peer-Reviewed Original ResearchTF RNA expressionHigh TF expressionTF expressionTissue factorEndothelial cellsChemical stimulationRNA expressionExcessive inflammatory reactionReal-time RT-PCRLaminar flowVascular endothelial cellsQuantitative real-time RT-PCRHemodynamic stabilizationIntravascular activationIntravascular thrombosisFro flowInflammatory reactionExtrinsic coagulationTumor growth
2011
Cyclic Strain Delays the Expression of Tissue Factor Induced by Thrombin in Human Umbilical Vein Endothelial Cells
Yamashita N, Abe R, Nixon A, Rochier A, Madri J, Sumpio B. Cyclic Strain Delays the Expression of Tissue Factor Induced by Thrombin in Human Umbilical Vein Endothelial Cells. International Journal Of Angiology 2011, 20: 157-166. PMID: 22942631, PMCID: PMC3331651, DOI: 10.1055/s-0031-1284475.Peer-Reviewed Original ResearchHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsVein endothelial cellsExtracellular signal-regulated protein kinase (ERK) inhibitorsProtein kinase inhibitorsEndothelial cellsEgr-1 levelsTF expressionERK activityERK inhibitorThrRNA expressionP38Messenger RNA expressionTF mRNA expressionCyclic strainCulture conditionsExpression of TFKinase inhibitorsExpressionStationary culture conditionsTissue factor expressionFactor expressionCellsMRNA expressionVarying Effects of Hemodynamic Forces on Tissue Factor RNA Expression in Human Endothelial Cells
Abe R, Yamashita N, Rochier A, Nixon A, Abe R, Madri JA, Sumpio BE. Varying Effects of Hemodynamic Forces on Tissue Factor RNA Expression in Human Endothelial Cells. Journal Of Surgical Research 2011, 170: 150-156. PMID: 21592524, DOI: 10.1016/j.jss.2011.04.002.Peer-Reviewed Original ResearchConceptsLaminar flowOscillatory flowCyclic strainUnidirectional laminar flowUniform laminar flowMechanical stressDisturbed flowTF RNA expressionHuman umbilical vein endothelial cellsFlowStatic controlHigh TF expressionTF expressionForceMechanical forcesEndothelial cellsRNA expressionSustained amplification
2010
The Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty
Basco MT, Yiu WK, Cheng SW, Sumpio BE. The Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty. Journal Of Vascular And Interventional Radiology 2010, 21: 910-915. PMID: 20417120, PMCID: PMC2878641, DOI: 10.1016/j.jvir.2010.02.016.Peer-Reviewed Original Research
2008
Synergistic Effect of Cool/Thaw Cycles on Vascular Cells in an In Vitro Model of Cryoplasty
Yiu WK, Cheng SW, Sumpio BE. Synergistic Effect of Cool/Thaw Cycles on Vascular Cells in an In Vitro Model of Cryoplasty. Journal Of Vascular And Interventional Radiology 2008, 19: 925-930. PMID: 18503909, DOI: 10.1016/j.jvir.2008.02.007.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsAkt activationEndothelial cellsApoptotic smooth muscle cellsActivation of AktBovine aortic smooth muscle cellsAortic smooth muscle cellsSurvival responseDeoxynucleotidyl transferase-mediated dUTP nick end labelingFetal bovine serumTerminal deoxynucleotidyl transferase-mediated dUTP nick end labelingTransferase-mediated dUTP nick end labelingImmunoblot analysisDUTP nick end labelingHigher apoptotic rateVascular cellsQS163. Differential Effects on Endothelial Cells and Smooth Muscle Cells After Freezing and Rewarming
Basco M, Yiu W, Sumpio B. QS163. Differential Effects on Endothelial Cells and Smooth Muscle Cells After Freezing and Rewarming. Journal Of Surgical Research 2008, 144: 332. DOI: 10.1016/j.jss.2007.12.405.Peer-Reviewed Original ResearchDifferential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells
Kim JI, Cordova AC, Hirayama Y, Madri JA, Sumpio BE. Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells. Endothelium 2008, 15: 33-42. PMID: 18568943, PMCID: PMC2644408, DOI: 10.1080/10623320802092260.Peer-Reviewed Original ResearchConceptsSp1 phosphorylationMT1-MMP expressionPromoter sitesPKCzeta inhibitorProtein kinase CzetaAffinity of Sp1Egr-1 bindingProtein kinase CζExtracellular matrix remodelingEndothelial cell migrationSp1Cell migrationPhosphorylationMatrix remodelingProtein expressionCyclic strainExpressionMembrane typeEndothelial cellsKey roleCzetaInhibitorsCζMetalloproteinaseAffinity
2007
Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain
Hoshino Y, Nishimura K, Sumpio BE. Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain. Journal Of Cellular Biochemistry 2007, 100: 515-526. PMID: 16927376, DOI: 10.1002/jcb.21085.Peer-Reviewed Original ResearchConceptsCasein kinase 2Transfection of ECsPTEN plasmidVascular cell morphologyEndothelial cellsPI3K activitySuppression of apoptosisPI3K-Akt pathwayLipid phosphataseMaximal activityPhosphatase PTENPhospho-PTENPTEN activityBovine aortic endothelial cellsIntracellular phosphatidylinositolUpstream regulatorAkt activityKinase 2Phospho-AKT activityK activityTime-dependent mannerAortic endothelial cellsCell morphologyCell proliferationPTENP139 Differential survival responses of vascular smooth muscle cells and endothelial cells towards different cycles of supercooling and rewarming in a model simulating cryoplasty
Yiu W, Cheng S, Sumpio B. P139 Differential survival responses of vascular smooth muscle cells and endothelial cells towards different cycles of supercooling and rewarming in a model simulating cryoplasty. Journal Of Surgical Research 2007, 137: 292. DOI: 10.1016/j.jss.2006.12.370.Peer-Reviewed Original ResearchRole of Ligand-Specific Integrins in Endothelial Cell Alignment and Elongation Induced by Cyclic Strain
Hirayama Y, Sumpio BE. Role of Ligand-Specific Integrins in Endothelial Cell Alignment and Elongation Induced by Cyclic Strain. Endothelium 2007, 14: 275-283. PMID: 18080865, DOI: 10.1080/10623320701746248.Peer-Reviewed Original ResearchConceptsEndothelial cellsP38 MAPKAnti-alpha2 integrin antibodyMorphological changesP38 mitogen-activated protein kinase (MAPK) pathwayCycles/minIntegrin-blocking antibodiesP38 MAPK pathwayMitogen-activated protein kinase pathwayAlpha 5Integrin antibodyProtein kinase pathwayMAPK pathwayAntibodiesKinase pathwayMAPKMultiple biological processesActivationIntegrinsCritical roleCyclic strainPathway
2006
PECAM-1 Affects GSK-3β-Mediated β-Catenin Phosphorylation and Degradation
Biswas P, Canosa S, Schoenfeld D, Schoenfeld J, Li P, Cheas LC, Zhang J, Cordova A, Sumpio B, Madri JA. PECAM-1 Affects GSK-3β-Mediated β-Catenin Phosphorylation and Degradation. American Journal Of Pathology 2006, 169: 314-324. PMID: 16816383, PMCID: PMC1698776, DOI: 10.2353/ajpath.2006.051112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta CateninBlotting, WesternCapillary PermeabilityCells, CulturedEndothelial CellsFluorescent Antibody TechniqueGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHistamineHistamine AgentsHumansMiceModels, BiologicalPhosphatidylinositol 3-KinasesPhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Proto-Oncogene Proteins c-aktReceptors, HistamineSignal TransductionConceptsAdherens junctionsSerine phosphorylationSrc homology 2 domainBeta-catenin expression levelsAdherens junction componentsSerine phosphorylation levelEndothelial cellsΒ-catenin phosphorylationPECAM-1Cell biological responsesCytoplasmic domainSHP-2Proteosomal degradationGSK-3betaDynamic regulatorJunction componentsPhosphorylation levelsPhosphorylationEndothelial cell adhesion molecule-1Expression levelsGSK-3βBiological responsesEndothelial barrier permeabilityMice exhibitCell adhesion molecule-1Role of AKT in cyclic strain-induced endothelial cell proliferation and survival
Nishimura K, Li W, Hoshino Y, Kadohama T, Asada H, Ohgi S, Sumpio BE. Role of AKT in cyclic strain-induced endothelial cell proliferation and survival. American Journal Of Physiology - Cell Physiology 2006, 290: c812-c821. PMID: 16469863, DOI: 10.1152/ajpcell.00347.2005.Peer-Reviewed Original ResearchConceptsEndothelial cellsCycles/minRole of AktEC proliferationCultured bovine aortic endothelial cellsSurvival of ECsAortic endothelial cellsApoptotic endothelial cellsGSK-3betaBovine aortic endothelial cellsEndothelial cell proliferationFrequency of strainsPositive cellsGlycogen synthase kinaseKinase-dead AktCell proliferationCaspase-3AktSurvivalCell numberSynthase kinaseProliferationEarly phosphorylationBad phosphorylationNicotine enhances human vascular endothelial cell expression of ICAM-1 and VCAM-1 via protein kinase C, p38 mitogen-activated protein kinase, NF-κB, and AP-1
Ueno H, Pradhan S, Schlessel D, Hirasawa H, Sumpio BE. Nicotine enhances human vascular endothelial cell expression of ICAM-1 and VCAM-1 via protein kinase C, p38 mitogen-activated protein kinase, NF-κB, and AP-1. Cardiovascular Toxicology 2006, 6: 39-50. PMID: 16845181, DOI: 10.1385/ct:6:1:39.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsICAM-1VCAM-1NF-κBAdhesion moleculesP38 mitogen-activated protein kinaseProtein kinase CMitogen-activated protein kinaseVascular cell adhesion moleculeEndothelial cellsExposure of HUVECsCellular adhesion molecule expressionAP-1Vascular endothelial cell expressionMajor risk factorEffects of nicotineEtiology of atherosclerosisP38 MAPKAdhesion molecule expressionIntercellular adhesion moleculeTranscription factor NF-κBCellular adhesion moleculesEndothelial cell expressionFactor NF-κBUmbilical vein endothelial cellsp38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress
Kadohama T, Akasaka N, Nishimura K, Hoshino Y, Sasajima T, Sumpio BE. p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress. Endothelium 2006, 13: 43-50. PMID: 16885066, DOI: 10.1080/10623320600660219.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAtherosclerosisBlood PressureBlood VesselsCattleCell PolarityCell ShapeCells, CulturedDose-Response Relationship, DrugEndothelial CellsEnzyme ActivationEnzyme InhibitorsHemodynamicsMAP Kinase Signaling SystemP38 Mitogen-Activated Protein KinasesPhosphorylationRegional Blood FlowStress, MechanicalTime FactorsConceptsStatic endothelial cellsFluid shear stressP38 MAPKP38 Mitogen-Activated Protein Kinase ActivationMitogen-Activated Protein Kinase ActivationEndothelial cellsProtein kinase activationP38 MAPK inhibitor SB-203580Inhibitor SB 203580Cell alignmentProtein kinaseBovine aortic endothelial cellsLaminar shear stressKinase activationSB 203580P38 mitogenCultured bovine aortic endothelial cellsEC elongationAortic endothelial cellsMAPKCellsElongationActivationMorphometric analysisKinase