2007
Role of Ligand-Specific Integrins in Endothelial Cell Alignment and Elongation Induced by Cyclic Strain
Hirayama Y, Sumpio BE. Role of Ligand-Specific Integrins in Endothelial Cell Alignment and Elongation Induced by Cyclic Strain. Endothelium 2007, 14: 275-283. PMID: 18080865, DOI: 10.1080/10623320701746248.Peer-Reviewed Original ResearchConceptsEndothelial cellsP38 MAPKAnti-alpha2 integrin antibodyMorphological changesP38 mitogen-activated protein kinase (MAPK) pathwayCycles/minIntegrin-blocking antibodiesP38 MAPK pathwayMitogen-activated protein kinase pathwayAlpha 5Integrin antibodyProtein kinase pathwayMAPK pathwayAntibodiesKinase pathwayMAPKMultiple biological processesActivationIntegrinsCritical roleCyclic strainPathwayRole of ligand specific integrins in endothelial cell alignment and elongation induced by cyclic strain
HIRAYAMA Y, Sumpio B. Role of ligand specific integrins in endothelial cell alignment and elongation induced by cyclic strain. The FASEB Journal 2007, 21: a752-a752. DOI: 10.1096/fasebj.21.6.a752-b.Peer-Reviewed Original Research
2006
Role of AKT in cyclic strain-induced endothelial cell proliferation and survival
Nishimura K, Li W, Hoshino Y, Kadohama T, Asada H, Ohgi S, Sumpio BE. Role of AKT in cyclic strain-induced endothelial cell proliferation and survival. American Journal Of Physiology - Cell Physiology 2006, 290: c812-c821. PMID: 16469863, DOI: 10.1152/ajpcell.00347.2005.Peer-Reviewed Original ResearchConceptsEndothelial cellsCycles/minRole of AktEC proliferationCultured bovine aortic endothelial cellsSurvival of ECsAortic endothelial cellsApoptotic endothelial cellsGSK-3betaBovine aortic endothelial cellsEndothelial cell proliferationFrequency of strainsPositive cellsGlycogen synthase kinaseKinase-dead AktCell proliferationCaspase-3AktSurvivalCell numberSynthase kinaseProliferationEarly phosphorylationBad phosphorylation
2004
Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway
Li W, Sumpio BE. Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway. AJP Heart And Circulatory Physiology 2004, 288: h1591-h1597. PMID: 15591103, DOI: 10.1152/ajpheart.00382.2004.Peer-Reviewed Original ResearchMeSH Keywords3-Phosphoinositide-Dependent Protein KinasesAndrostadienesAnimalsAntibiotics, AntineoplasticAortaCarrier ProteinsCattleCell DivisionCells, CulturedChromonesEndothelium, VascularEnzyme InhibitorsFlavonoidsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MorpholinesPhosphatidylinositol 3-KinasesPhosphodiesterase InhibitorsPhosphoinositide-3 Kinase InhibitorsPhosphoproteinsPhosphorylationProtein KinasesProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRibosomal Protein S6 KinasesSignal TransductionSirolimusTOR Serine-Threonine KinasesWortmanninConceptsVascular endothelial cell proliferationEndothelial cell proliferationStrain-induced activationSignal pathwayEC proliferationPD 98059Cell proliferationPI3K inhibitor wortmanninPI3K inhibitorsCycles/minExtracellular signal-regulated kinases 1Inhibitor PD 98059MTOR pathwaySignal-regulated kinases 1Bovine aortic ECsMammalian targetMTOR-4EK inhibitorsEukaryotic initiation factor 4EAortic ECsInitiation factor 4EMEK1 inhibitor PD 98059K activationProliferationRapamycin
2000
Role of Caveolin in Hemodynamic Force-Mediated Endothelial Changes
Fujioka K, Azuma N, Kito H, Gahtan V, Esato K, Sumpio B. Role of Caveolin in Hemodynamic Force-Mediated Endothelial Changes. Journal Of Surgical Research 2000, 92: 7-10. PMID: 10864474, DOI: 10.1006/jsre.2000.5838.Peer-Reviewed Original ResearchTranslocation of PKC isoforms in bovine aortic smooth muscle cells exposed to strain
Han O, Takei T, Basson M, Sumpio B. Translocation of PKC isoforms in bovine aortic smooth muscle cells exposed to strain. Journal Of Cellular Biochemistry 2000, 80: 367-372. PMID: 11135367, DOI: 10.1002/1097-4644(20010301)80:3<367::aid-jcb100>3.0.co;2-2.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsAortic smooth muscle cellsBovine aortic smooth muscle cellsMuscle cellsPKC-alphaPKC isoformsCyclic strain resultsCycles/minCytosolic fractionProliferation responseParticulate fractionNF-kappaBProtein kinase C activityCollagen type ICellular proliferationSignificant stimulationImmunoblotting analysisKinase C activityType IPKC activityC activityStimulationIndividual PKC isoformsIsoforms
1998
Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain
Ikeda M, Takei T, Mills I, Sumpio B. Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain. Biochemical And Biophysical Research Communications 1998, 247: 462-465. PMID: 9642151, DOI: 10.1006/bbrc.1998.8811.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channel BlockersCalcium-Calmodulin-Dependent Protein KinasesCattleCells, CulturedChelating AgentsEgtazic AcidEndothelium, VascularEnzyme ActivationExtracellular SpaceGadoliniumHydroquinonesIntracellular FluidMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationStress, MechanicalConceptsEndothelial cellsActivation of ERK1/2Baseline phosphorylationExtracellular Ca2Normal extracellular Ca2Aortic endothelial cellsKinase 1Bovine aortic endothelial cellsStrain-induced activationCycles/minChannel blockersExtracellular signal-regulated kinases 1Signal-regulated kinases 1Calcium-independent activationBenzohydroquinoneERK1/2 activationERK1/2Free mediumEffect of Ca2ActivationCa2EGTACyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain
Kito H, Yokoyama C, Inoue H, Tanabe T, Nakajima N, Sumpio B. Cyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain. Endothelium 1998, 6: 107-112. PMID: 9930644, DOI: 10.3109/10623329809072197.Peer-Reviewed Original ResearchConceptsAortic endothelial cellsBovine aortic endothelial cellsEndothelial cellsInduction of COXCOX-1 expressionCOX-2 promoter activityExposure of ECsPromoter activityCycles/minCOX-2Cyclooxygenase expressionCOX-1Weak inducerMRNA expressionExtent of inductionNorthern blot analysisBlot analysisTransient transfection experimentsLipopolysaccharideMinimal effectExpressionInductionTransfection experimentsCyclic strainCells
1997
Exposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools
ROSALES O, ISALES C, BARRETT P, BROPHY C, SUMPIO B. Exposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools. Biochemical Journal 1997, 326: 385-392. PMID: 9291109, PMCID: PMC1218682, DOI: 10.1042/bj3260385.Peer-Reviewed Original ResearchConceptsMicroM GdCl3Endothelial cellsIP3 formationLate-phase componentsInitiation of stretchExtracellular poolConfluent bovine aortic endothelial cellsMobilization of intracellularAortic endothelial cellsBovine aortic endothelial cellsNM charybdotoxinCycles/minIP3 generationIntracellular Ca2Sustained phaseCytosolic Ca2Confluent endothelial cellsBenzohydroquinoneFree mediumTemporal relationshipMicroM NiCl2Ca2PreincubationCellsInitial riseCyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells
Yano Y, Geibel J, Sumpio B. Cyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells. Journal Of Cellular Biochemistry 1997, 64: 505-513. PMID: 9057108, DOI: 10.1002/(sici)1097-4644(19970301)64:3<505::aid-jcb17>3.0.co;2-e.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsBeta 1 integrinUmbilical vein endothelial cellsVein endothelial cellsEndothelial cellsAlpha 5Beta 1Alpha 5 beta 1Alpha 2 beta 1 integrinH exposureBeta 3 integrinAlpha 2 integrinCycles/minEndothelial cell morphologyVariety of stimuliIntegrin alpha 5Tyrosine phosphorylationVitronectin receptorAlpha 2Redistribution of alphaEC subjectsBeta integrins
1995
Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells.
Awolesi MA, Sessa WC, Sumpio BE. Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells. Journal Of Clinical Investigation 1995, 96: 1449-1454. PMID: 7544806, PMCID: PMC185768, DOI: 10.1172/jci118181.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseAortic endothelial cellsNitric oxide synthaseBovine aortic endothelial cellsCultured bovine aortic endothelial cellsEndothelial cellsOxide synthaseENOS transcriptsRegulation of eNOSENOS gene expressionBlot analysisHemodynamic forcesWestern blot analysisENOS levelsCycles/minImmunohistochemical stainingNitric oxideProtein levelsNorthern blot analysisNuclear runoff transcription assaysVivoCellsRunoff transcription assaysCytosolic RNAExpression
1994
Cyclic strain increases endothelial nitric oxide synthase activity.
Awolesi MA, Widmann MD, Sessa WC, Sumpio BE. Cyclic strain increases endothelial nitric oxide synthase activity. Surgery 1994, 116: 439-44; discussion 444-5. PMID: 7519368.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseRegulation of eNOSCultured bovine aortic endothelial cellsENOS activityAortic endothelial cellsEndothelial cellsBovine aortic endothelial cellsEndothelial nitric oxide synthase activityNitric oxideNitric oxide synthase activityOxide synthase activityNitric oxide synthaseNitric oxide productionHemodynamic forcesRelaxing factorsGreiss reactionCycles/minOxide synthaseENOS geneMedium of ECOxide productionCycles/minuteFunctional activityStrain regimensSynthase activityChronic Cyclic Strain Reduces Adenylate Cyclase Activity and Stimulatory G Protein Subunit Levels in Coronary Smooth Muscle Cells
Wiersbitzky M, Mills I, Sumpio B, Gewirtz H. Chronic Cyclic Strain Reduces Adenylate Cyclase Activity and Stimulatory G Protein Subunit Levels in Coronary Smooth Muscle Cells. Experimental Cell Research 1994, 210: 52-55. PMID: 8269996, DOI: 10.1006/excr.1994.1008.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsSmooth muscle cellsAdenylate cyclase activityCoronary vascular smooth muscle cellsMuscle cellsCyclase activityChronic cyclic strainG protein subunit levelsCoronary smooth muscle cellsProtein steady-state levelsCoronary smooth muscleCultured smooth muscle cellsSteady-state levelsPorcine coronary arteriesCoronary arteryCoronary circulationBeta levelsCycles/minProtein subunit levelsSmooth muscleGi alphaIntravascular pressureEffector pathwaysUnstretched cellsSubunit levels
1992
Protein kinase C is a mediator of the adaptation of vascular endothelial cells to cyclic strain in vitro.
Rosales OR, Sumpio BE. Protein kinase C is a mediator of the adaptation of vascular endothelial cells to cyclic strain in vitro. Surgery 1992, 112: 459-66. PMID: 1379381.Peer-Reviewed Original ResearchConceptsProtein kinase CActivation of PKCImmunocytochemical stainingPKC activityCyclic stretchEndothelial cell activationVascular endothelial cellsCycles/minSustained elevationCell activationFlexible-bottomed culture platesKinase CBovine aortic ECsECS resultsEndothelial cellsBlood vesselsAortic ECsEvidence of translocationSpecific PKC inhibitorEC proliferationCalphostin CEC growthHemodynamic forces
1990
Enhanced production of endothelium-derived contracting factor by endothelial cells subjected to pulsatile stretch.
Sumpio BE, Widmann MD. Enhanced production of endothelium-derived contracting factor by endothelial cells subjected to pulsatile stretch. Surgery 1990, 108: 277-81; discussion 281-2. PMID: 2200155.Peer-Reviewed Original ResearchMechanosensitive adenylate cyclase activity in coronary vascular smooth muscle cells
Mills I, Letsou G, Rabban J, Sumpio B, Gewirtz H. Mechanosensitive adenylate cyclase activity in coronary vascular smooth muscle cells. Biochemical And Biophysical Research Communications 1990, 171: 143-147. PMID: 2393388, DOI: 10.1016/0006-291x(90)91368-3.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsSmooth muscle cellsCoronary vascular smooth muscle cellsMuscle cellsCyclase activityPorcine coronary artery smooth muscle cellsCultured vascular smooth muscle cellsArtery smooth muscle cellsCoronary artery smooth muscle cellsVascular smooth muscleMagnitude of stretchStretch-induced reductionAdenylate cyclase activityPotential biochemical mechanismsPerfusion pressureCoronary circulationCycles/minMyogenic responseSmooth muscleMechanical stretchAdenylate cyclasePotential mechanismsEntire time courseUnstretched cellsTime course