2009
Lovastatin Inhibits Thrombospondin-1-Induced Smooth Muscle Cell Chemotaxis1
Esemuede N, Lee T, Maier KG, Sumpio BE, Gahtan V. Lovastatin Inhibits Thrombospondin-1-Induced Smooth Muscle Cell Chemotaxis1. Journal Of Surgical Research 2009, 168: 149-154. PMID: 20338582, DOI: 10.1016/j.jss.2009.11.728.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell ProliferationCells, CulturedChemotaxisDose-Response Relationship, DrugHydroxymethylglutaryl-CoA Reductase InhibitorsLovastatinMevalonic AcidModels, AnimalMonomeric GTP-Binding ProteinsMuscle, Smooth, VascularRas ProteinsSignal TransductionThrombospondin 1Tunica IntimaTunica MediaConceptsTSP-1-induced chemotaxisGeranylgeranyl transferase inhibitorSerum-free mediumThrombospondin-1HMG-CoA reductase inhibitorsVascular smooth muscle cell migrationSmooth muscle cell migrationTransferase inhibitorsEffect of lovastatinG proteinsMuscle cell migrationRas activationRho-kinase inhibitorCell migrationPost-hoc testingFarnesyl transferase inhibitorsCholesterol loweringLovastatin doseVascular restenosisIntimal hyperplasiaBoyden chamberPleiotropic propertiesReductase inhibitorsWestern blotInhibition of Ras
2005
Differential responsiveness of early- and late-passage endothelial cells to shear stress
Kudo FA, Warycha B, Juran PJ, Asada H, Teso D, Aziz F, Frattini J, Sumpio BE, Nishibe T, Cha C, Dardik A. Differential responsiveness of early- and late-passage endothelial cells to shear stress. The American Journal Of Surgery 2005, 190: 763-769. PMID: 16226955, DOI: 10.1016/j.amjsurg.2005.07.017.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAortaApoptosisBlotting, WesternCattleCell CountCell DivisionCell ProliferationCells, CulturedEndothelium, VascularIn Vitro TechniquesMuscle, Smooth, VascularPhosphorylationProliferating Cell Nuclear AntigenProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthStress, MechanicalTumor Suppressor Protein p53ConceptsLate passage endothelial cellsOrbital shear stressEarly passage cellsSmooth muscle cell migrationMuscle cell migrationEndothelial cellsSenescence modelAkt phosphorylationCell migrationProtein kinase B activationPassage cellsKinase B activationCell proliferationVascular disease increasesLate passage cellsBovine aortic endothelial cellsNuclear antigen reactivityAortic endothelial cellsEndothelial cell proliferationNeointimal hyperplasiaAntigen reactivityTotal AktBoyden chamberB activationWestern blottingHomocysteine promotes p38-dependent chemotaxis in bovine aortic smooth muscle cells
Akasaka K, Akasaka N, Di Luozzo G, Sasajima T, Sumpio BE. Homocysteine promotes p38-dependent chemotaxis in bovine aortic smooth muscle cells. Journal Of Vascular Surgery 2005, 41: 517-522. PMID: 15838488, DOI: 10.1016/j.jvs.2004.12.043.Peer-Reviewed Original ResearchConceptsEffect of homocysteineMigration of SMCsLevels of homocysteineProgressive intimal thickeningAortic smooth muscle cellsSmooth muscle cell migrationBovine aortic smooth muscle cellsPotential therapeutic implicationsSmooth muscle cellsP38 activationExposure of SMCMuscle cell migrationSMC chemotaxisRisk factorsSelective blockadeIntimal thickeningTherapeutic implicationsP38-dependent pathwaySMC proliferationBoyden chamberChemotactic potentialMuscle cellsHomocysteineFetal bovine serumP38 inhibitor
2003
Shear stress stimulated endothelial cell derived PDGF and IL-1 alpha both stimulate SMC chemotaxis via the MAPK pathway
Dardik A, Yamashita A, Aziz F, Paszkowiak J, Asada H, Sumpio B. Shear stress stimulated endothelial cell derived PDGF and IL-1 alpha both stimulate SMC chemotaxis via the MAPK pathway. Journal Of Surgical Research 2003, 114: 249. DOI: 10.1016/j.jss.2003.08.159.Peer-Reviewed Original ResearchPlatelet-derived growth factor-BBIL-1SMC migrationEndothelial cellsMAPK inhibitor PD98059Pathogenesis of atherosclerosisSMC chemotaxisSmooth muscle cell migrationIL-1 alphaInterleukin-1 alphaSimilar degreeInhibitor PD98059Muscle cell migrationMAPK pathwayHemodynamic forcesGrowth factor-BBAortic endothelial cellsSS stimulationBovine aortic endothelial cellsArterial levelsNeointimal hyperplasiaParacrine mechanismsSMC mitogenBoyden chamberSoluble factors