2010
The Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty
Basco MT, Yiu WK, Cheng SW, Sumpio BE. The Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty. Journal Of Vascular And Interventional Radiology 2010, 21: 910-915. PMID: 20417120, PMCID: PMC2878641, DOI: 10.1016/j.jvir.2010.02.016.Peer-Reviewed Original Research
2007
Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain
Hoshino Y, Nishimura K, Sumpio BE. Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain. Journal Of Cellular Biochemistry 2007, 100: 515-526. PMID: 16927376, DOI: 10.1002/jcb.21085.Peer-Reviewed Original ResearchConceptsCasein kinase 2Transfection of ECsPTEN plasmidVascular cell morphologyEndothelial cellsPI3K activitySuppression of apoptosisPI3K-Akt pathwayLipid phosphataseMaximal activityPhosphatase PTENPhospho-PTENPTEN activityBovine aortic endothelial cellsIntracellular phosphatidylinositolUpstream regulatorAkt activityKinase 2Phospho-AKT activityK activityTime-dependent mannerAortic endothelial cellsCell morphologyCell proliferationPTEN
2006
Role of AKT in cyclic strain-induced endothelial cell proliferation and survival
Nishimura K, Li W, Hoshino Y, Kadohama T, Asada H, Ohgi S, Sumpio BE. Role of AKT in cyclic strain-induced endothelial cell proliferation and survival. American Journal Of Physiology - Cell Physiology 2006, 290: c812-c821. PMID: 16469863, DOI: 10.1152/ajpcell.00347.2005.Peer-Reviewed Original ResearchConceptsEndothelial cellsCycles/minRole of AktEC proliferationCultured bovine aortic endothelial cellsSurvival of ECsAortic endothelial cellsApoptotic endothelial cellsGSK-3betaBovine aortic endothelial cellsEndothelial cell proliferationFrequency of strainsPositive cellsGlycogen synthase kinaseKinase-dead AktCell proliferationCaspase-3AktSurvivalCell numberSynthase kinaseProliferationEarly phosphorylationBad phosphorylationp38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress
Kadohama T, Akasaka N, Nishimura K, Hoshino Y, Sasajima T, Sumpio BE. p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress. Endothelium 2006, 13: 43-50. PMID: 16885066, DOI: 10.1080/10623320600660219.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAtherosclerosisBlood PressureBlood VesselsCattleCell PolarityCell ShapeCells, CulturedDose-Response Relationship, DrugEndothelial CellsEnzyme ActivationEnzyme InhibitorsHemodynamicsMAP Kinase Signaling SystemP38 Mitogen-Activated Protein KinasesPhosphorylationRegional Blood FlowStress, MechanicalTime FactorsConceptsStatic endothelial cellsFluid shear stressP38 MAPKP38 Mitogen-Activated Protein Kinase ActivationMitogen-Activated Protein Kinase ActivationEndothelial cellsProtein kinase activationP38 MAPK inhibitor SB-203580Inhibitor SB 203580Cell alignmentProtein kinaseBovine aortic endothelial cellsLaminar shear stressKinase activationSB 203580P38 mitogenCultured bovine aortic endothelial cellsEC elongationAortic endothelial cellsMAPKCellsElongationActivationMorphometric analysisKinase
2005
Differential responsiveness of early- and late-passage endothelial cells to shear stress
Kudo FA, Warycha B, Juran PJ, Asada H, Teso D, Aziz F, Frattini J, Sumpio BE, Nishibe T, Cha C, Dardik A. Differential responsiveness of early- and late-passage endothelial cells to shear stress. The American Journal Of Surgery 2005, 190: 763-769. PMID: 16226955, DOI: 10.1016/j.amjsurg.2005.07.017.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAortaApoptosisBlotting, WesternCattleCell CountCell DivisionCell ProliferationCells, CulturedEndothelium, VascularIn Vitro TechniquesMuscle, Smooth, VascularPhosphorylationProliferating Cell Nuclear AntigenProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthStress, MechanicalTumor Suppressor Protein p53ConceptsLate passage endothelial cellsOrbital shear stressEarly passage cellsSmooth muscle cell migrationMuscle cell migrationEndothelial cellsSenescence modelAkt phosphorylationCell migrationProtein kinase B activationPassage cellsKinase B activationCell proliferationVascular disease increasesLate passage cellsBovine aortic endothelial cellsNuclear antigen reactivityAortic endothelial cellsEndothelial cell proliferationNeointimal hyperplasiaAntigen reactivityTotal AktBoyden chamberB activationWestern blotting
2004
17β-Estradiol Exposure Leads to Activation of ERK and p38 but Not JNK in Vascular Endothelial Cells
Knox R, Chen E, Wang X, Jeffries B, Yamaguchi M, Kim S, Sumpio B. 17β-Estradiol Exposure Leads to Activation of ERK and p38 but Not JNK in Vascular Endothelial Cells. International Journal Of Angiology 2004, 13: 67-70. DOI: 10.1007/s00547-004-1068-9.Peer-Reviewed Original ResearchEndothelial nitric oxide synthaseVascular endothelial cellsBovine aortic endothelial cellsEndothelial cellsNitric oxideNitric oxide synthaseEndothelial NO productionAnti-phosphospecific antibodiesEndothelial cell productionAortic endothelial cellsRapid activationAtheroprotective effectsActivation of ERKOxide synthaseFetal calf serumE2 exposureNO productionENOS activationActivity of JNKEstrogenBiphasic patternP38 phosphorylationMonophasic mannerTotal ERKERK phosphorylation17β-Estradiol exposure leads to activation of ERK and p38 but not JNK in vascular endothelial cells
Knox R, Chen E, Wang X, Jeffries B, Yamaguchi M, Kim S, Sumpio B. 17β-Estradiol exposure leads to activation of ERK and p38 but not JNK in vascular endothelial cells. International Journal Of Angiology 2004, 67-70. DOI: 10.1055/s-0031-1276450.Peer-Reviewed Original ResearchEndothelial nitric oxide synthaseVascular endothelial cellsBovine aortic endothelial cellsEndothelial cellsNitric oxideNitric oxide synthaseEndothelial NO productionAnti-phosphospecific antibodiesEndothelial cell productionAortic endothelial cellsRapid activationAtheroprotective effectsActivation of ERKOxide synthaseFetal calf serumE2 exposureNO productionENOS activationActivity of JNKEstrogenBiphasic patternP38 phosphorylationMonophasic mannerTotal ERKERK phosphorylation
2003
Shear stress stimulated endothelial cell derived PDGF and IL-1 alpha both stimulate SMC chemotaxis via the MAPK pathway
Dardik A, Yamashita A, Aziz F, Paszkowiak J, Asada H, Sumpio B. Shear stress stimulated endothelial cell derived PDGF and IL-1 alpha both stimulate SMC chemotaxis via the MAPK pathway. Journal Of Surgical Research 2003, 114: 249. DOI: 10.1016/j.jss.2003.08.159.Peer-Reviewed Original ResearchPlatelet-derived growth factor-BBIL-1SMC migrationEndothelial cellsMAPK inhibitor PD98059Pathogenesis of atherosclerosisSMC chemotaxisSmooth muscle cell migrationIL-1 alphaInterleukin-1 alphaSimilar degreeInhibitor PD98059Muscle cell migrationMAPK pathwayHemodynamic forcesGrowth factor-BBAortic endothelial cellsSS stimulationBovine aortic endothelial cellsArterial levelsNeointimal hyperplasiaParacrine mechanismsSMC mitogenBoyden chamberSoluble factorsShear Stress and Cyclic Strain May Suppress Apoptosis in Endothelial Cells by Different Pathways
Haga M, Chen A, Gortler D, Dardik A, Sumpio B. Shear Stress and Cyclic Strain May Suppress Apoptosis in Endothelial Cells by Different Pathways. Endothelium 2003, 10: 149-157. DOI: 10.1080/713715223.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBcl-Associated Death ProteinCarrier ProteinsCattleCells, CulturedCulture Media, Serum-FreeEndothelial CellsEnzyme InhibitorsHemodynamicsPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktSignal TransductionStress, MechanicalTranscription, GeneticConceptsPhosphorylation of AktEndothelial cellsPI3K inhibitionInhibition of apoptosisK inhibitionHemodynamic forcesAortic endothelial cellsBovine aortic endothelial cellsEffect of SSArterial levelsIntimal hyperplasiaDownstream target BadNormal endotheliumMaximal stimulationAkt phosphorylationLaminar shear stressApoptosisDownstream phosphorylationAktInhibitionCell survivalEC growthSerum withdrawalAlternate pathwayPhosphorylation of BadRole of PP2A in the regulation of p38 MAPK activation in bovine aortic endothelial cells exposed to cyclic strain
Lee T, Kim SJ, Sumpio BE. Role of PP2A in the regulation of p38 MAPK activation in bovine aortic endothelial cells exposed to cyclic strain. Journal Of Cellular Physiology 2003, 194: 349-355. PMID: 12548554, DOI: 10.1002/jcp.10211.Peer-Reviewed Original ResearchConceptsP38 MAPK activationMAPK activationCatalytic subunitBovine aortic endothelial cellsPhospho-p38 MAPKOkadaic acidProtein phosphatase type 2AInhibitor of PP2AAortic endothelial cellsEndothelial cellsProtein kinaseRapid phosphorylationPP2AP38 mitogenRegulatory roleCell lysatesPhosphatase activityPP2AcMAPKPhosphorylationCyclic strainSubunitsType 2ACyclic strain resultsCellsShear Stress and Cyclic Strain May Suppress Apoptosis in Endothelial Cells by Different Pathways
Haga M, Chen A, Gortler D, Dardik A, Sumpio B. Shear Stress and Cyclic Strain May Suppress Apoptosis in Endothelial Cells by Different Pathways. Endothelium 2003, 10: 149-157. PMID: 13129818, DOI: 10.1080/10623320390233463.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBcl-Associated Death ProteinCarrier ProteinsCattleCells, CulturedCulture Media, Serum-FreeEndothelial CellsEnzyme InhibitorsHemodynamicsPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktSignal TransductionStress, MechanicalTranscription, GeneticConceptsPhosphorylation of AktEndothelial cellsPI3K inhibitionInhibition of apoptosisK inhibitionHemodynamic forcesAortic endothelial cellsBovine aortic endothelial cellsEffect of SSArterial levelsIntimal hyperplasiaDownstream target BadNormal endotheliumMaximal stimulationAkt phosphorylationLaminar shear stressApoptosisDownstream phosphorylationAktInhibitionCell survivalEC growthSerum withdrawalAlternate pathwayPhosphorylation of Bad
2000
Role of Caveolin in Hemodynamic Force-Mediated Endothelial Changes
Fujioka K, Azuma N, Kito H, Gahtan V, Esato K, Sumpio B. Role of Caveolin in Hemodynamic Force-Mediated Endothelial Changes. Journal Of Surgical Research 2000, 92: 7-10. PMID: 10864474, DOI: 10.1006/jsre.2000.5838.Peer-Reviewed Original Research
1998
Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain
Ikeda M, Takei T, Mills I, Sumpio B. Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain. Biochemical And Biophysical Research Communications 1998, 247: 462-465. PMID: 9642151, DOI: 10.1006/bbrc.1998.8811.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channel BlockersCalcium-Calmodulin-Dependent Protein KinasesCattleCells, CulturedChelating AgentsEgtazic AcidEndothelium, VascularEnzyme ActivationExtracellular SpaceGadoliniumHydroquinonesIntracellular FluidMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationStress, MechanicalConceptsEndothelial cellsActivation of ERK1/2Baseline phosphorylationExtracellular Ca2Normal extracellular Ca2Aortic endothelial cellsKinase 1Bovine aortic endothelial cellsStrain-induced activationCycles/minChannel blockersExtracellular signal-regulated kinases 1Signal-regulated kinases 1Calcium-independent activationBenzohydroquinoneERK1/2 activationERK1/2Free mediumEffect of Ca2ActivationCa2EGTACyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain
Kito H, Yokoyama C, Inoue H, Tanabe T, Nakajima N, Sumpio B. Cyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain. Endothelium 1998, 6: 107-112. PMID: 9930644, DOI: 10.3109/10623329809072197.Peer-Reviewed Original ResearchConceptsAortic endothelial cellsBovine aortic endothelial cellsEndothelial cellsInduction of COXCOX-1 expressionCOX-2 promoter activityExposure of ECsPromoter activityCycles/minCOX-2Cyclooxygenase expressionCOX-1Weak inducerMRNA expressionExtent of inductionNorthern blot analysisBlot analysisTransient transfection experimentsLipopolysaccharideMinimal effectExpressionInductionTransfection experimentsCyclic strainCells
1997
Exposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools
ROSALES O, ISALES C, BARRETT P, BROPHY C, SUMPIO B. Exposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools. Biochemical Journal 1997, 326: 385-392. PMID: 9291109, PMCID: PMC1218682, DOI: 10.1042/bj3260385.Peer-Reviewed Original ResearchConceptsMicroM GdCl3Endothelial cellsIP3 formationLate-phase componentsInitiation of stretchExtracellular poolConfluent bovine aortic endothelial cellsMobilization of intracellularAortic endothelial cellsBovine aortic endothelial cellsNM charybdotoxinCycles/minIP3 generationIntracellular Ca2Sustained phaseCytosolic Ca2Confluent endothelial cellsBenzohydroquinoneFree mediumTemporal relationshipMicroM NiCl2Ca2PreincubationCellsInitial rise
1996
Protein phosphatase 2A in stretch‐induced endothelial cell proliferation
Murata K, Mills I, Sumpio B. Protein phosphatase 2A in stretch‐induced endothelial cell proliferation. Journal Of Cellular Biochemistry 1996, 63: 311-319. PMID: 8913882, DOI: 10.1002/(sici)1097-4644(19961201)63:3<311::aid-jcb6>3.0.co;2-#.Peer-Reviewed Original ResearchConceptsProtein phosphatase 2APhosphatase 2AProtein phosphatase 2A activityProtein phosphatase 1Phosphatase 2A activityProtein kinase CPhosphatase 1Bovine aortic endothelial cellsOkadaic acidGrowth suppressorEndothelial cellsKinase CEndothelial cell proliferationCell growthFetal bovine serumCellular proliferationAortic endothelial cellsCell proliferationCyclic strainBovine serumKey mechanismProliferationCellsStrainsSuppressorTyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain
Yano Y, Geibel J, Sumpio BE. Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain. American Journal Of Physiology 1996, 271: c635-c649. PMID: 8770005, DOI: 10.1152/ajpcell.1996.271.2.c635.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell Adhesion MoleculesCell MovementCells, CulturedCytoskeletal ProteinsEndothelium, VascularFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGenisteinIsoflavonesNitrilesPaxillinPhosphoproteinsPhosphorylationPhosphotyrosineProtein-Tyrosine KinasesStress, MechanicalTissue DistributionTyrosineTyrphostinsTranscriptional regulation of endothelin.
Koo JT, Sawyer MD, Powell RJ, Sumpio BE. Transcriptional regulation of endothelin. Surgical Technology Online 1996, 5: 276-82. PMID: 15858752.Peer-Reviewed Original Research
1995
Cyclic strain causes heterogeneous induction of transcription factors, AP-1, CRE binding protein and NF-kB, in endothelial cells: Species and vascular bed diversity
Du W, Mills I, Sumpio B. Cyclic strain causes heterogeneous induction of transcription factors, AP-1, CRE binding protein and NF-kB, in endothelial cells: Species and vascular bed diversity. Journal Of Biomechanics 1995, 28: 1485-1491. PMID: 8666588, DOI: 10.1016/0021-9290(95)00096-8.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2AnimalsAortaCattleCell DivisionCyclic AMP Response Element-Binding ProteinEndothelium, VascularEpoprostenolGene Expression RegulationGenes, Immediate-EarlyHumansNF-kappa BNuclear ProteinsPhenotypeProtein Kinase CSpecies SpecificityStress, MechanicalTime FactorsTranscription Factor AP-1Transcription FactorsUmbilical VeinsConceptsHuman umbilical vein endothelial cellsBovine aortic endothelial cellsActivator protein-1NF-kBEndothelial cellsVascular bedDifferent vascular bedsUmbilical vein endothelial cellsAortic endothelial cellsCultured endothelial cellsTranscription factor activator protein-1Vein endothelial cellsProstacyclin secretionStretch-induced activationImmediate early genesEC exposureNuclear factorTime pointsHeterogeneous inductionCAMP response elementProtein 1Biphasic mannerSignificant inductionDivergent effectsHuman aortaCyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells.
Awolesi MA, Sessa WC, Sumpio BE. Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells. Journal Of Clinical Investigation 1995, 96: 1449-1454. PMID: 7544806, PMCID: PMC185768, DOI: 10.1172/jci118181.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseAortic endothelial cellsNitric oxide synthaseBovine aortic endothelial cellsCultured bovine aortic endothelial cellsEndothelial cellsOxide synthaseENOS transcriptsRegulation of eNOSENOS gene expressionBlot analysisHemodynamic forcesWestern blot analysisENOS levelsCycles/minImmunohistochemical stainingNitric oxideProtein levelsNorthern blot analysisNuclear runoff transcription assaysVivoCellsRunoff transcription assaysCytosolic RNAExpression