2020
Aberrantly elevated suprabasin in the bone marrow as a candidate biomarker of advanced disease state in myelodysplastic syndromes
Pribyl M, Hubackova S, Moudra A, Vancurova M, Polackova H, Stopka T, Jonasova A, Bokorova R, Fuchs O, Stritesky J, Salovska B, Bartek J, Hodny Z. Aberrantly elevated suprabasin in the bone marrow as a candidate biomarker of advanced disease state in myelodysplastic syndromes. Molecular Oncology 2020, 14: 2403-2419. PMID: 32696549, PMCID: PMC7530796, DOI: 10.1002/1878-0261.12768.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, DifferentiationAzacitidineBiomarkersBone MarrowCell CompartmentationCell Line, TumorChemokine CCL2Gene Expression Regulation, NeoplasticHEK293 CellsHumansInterferon-gammaLeukocytes, MononuclearLymphocyte CountMyelodysplastic SyndromesMyeloid CellsNeoplasm ProteinsPrognosisProto-Oncogene MasRNA, MessengerConceptsMyelodysplastic syndromeBone marrowMDS patientsPoor prognosisDisease progressionCandidate biomarkersHigh-risk myelodysplastic syndromeMyeloid-derived suppressor cellsAberrant cytokine levelsAdvanced disease stateMyeloid cell subpopulationsBlast percentageChemokine levelsCytokine levelsLymphocyte countSuppressor cellsPatient groupPeripheral bloodBM plasmaPreleukemic disordersImmune responseProinflammatory signalingMalignant cloneTherapy resistanceLeukemic cells
2019
Interferon‐regulated suprabasin is essential for stress‐induced stem‐like cell conversion and therapy resistance of human malignancies
Hubackova S, Pribyl M, Kyjacova L, Moudra A, Dzijak R, Salovska B, Strnad H, Tambor V, Imrichova T, Svec J, Vodicka P, Vaclavikova R, Rob L, Bartek J, Hodny Z. Interferon‐regulated suprabasin is essential for stress‐induced stem‐like cell conversion and therapy resistance of human malignancies. Molecular Oncology 2019, 13: 1467-1489. PMID: 30919591, PMCID: PMC6599850, DOI: 10.1002/1878-0261.12480.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinase/ERK kinaseSiRNA-mediated knockdownSBSN expressionERK pathwayProtein kinase/ERK kinaseHundreds of genesExtracellular signal-regulated kinase 1/2Signal-regulated kinase 1/2MEK/ERK pathwayCancer cellsPhenotypic plasticityTranscript profilesStress resistanceTherapy resistanceERK kinaseStem-like cellsActive NotchStem cell markersMolecular mechanismsAnoikis resistanceKinase 1/2Cancer evolutionChemical inhibitionCancer cell linesHuman clinical specimens