2019
Combining Rapid Data Independent Acquisition and CRISPR Gene Deletion for Studying Potential Protein Functions: A Case of HMGN1
Mehnert M, Li W, Wu C, Salovska B, Liu Y. Combining Rapid Data Independent Acquisition and CRISPR Gene Deletion for Studying Potential Protein Functions: A Case of HMGN1. Proteomics 2019, 19: e1800438. PMID: 30901150, DOI: 10.1002/pmic.201800438.Peer-Reviewed Original ResearchConceptsChromosomal protein HMG-14DIA-MSDIA mass spectrometryPotential protein functionsCRISPR-Cas gene editingImmune regulation processesCancer cellsExtracellular proteomeChromatin structureHistone inactivationFunctional annotationProtein functionCellular functionsRegulation eventsImportant functional implicationsHMG 14Gene knockoutEnrichment analysisData-independent acquisitionHMGN1Protein deletionCRISPR experimentsGene editingStress pathwaysIndependent acquisition
2017
Analysis of proteomes released from in vitro cultured eight Clostridium difficile PCR ribotypes revealed specific expression in PCR ribotypes 027 and 176 confirming their genetic relatedness and clinical importance at the proteomic level
Dresler J, Krutova M, Fucikova A, Klimentova J, Hruzova V, Duracova M, Houdkova K, Salovska B, Matejkova J, Hubalek M, Pajer P, Pisa L, Nyc O. Analysis of proteomes released from in vitro cultured eight Clostridium difficile PCR ribotypes revealed specific expression in PCR ribotypes 027 and 176 confirming their genetic relatedness and clinical importance at the proteomic level. Gut Pathogens 2017, 9: 45. PMID: 28814976, PMCID: PMC5556371, DOI: 10.1186/s13099-017-0194-9.Peer-Reviewed Original ResearchFlagellar proteinsProteomic levelToxin AClinical importanceLife-threatening toxic megacolonPCR ribotypesHistidine synthesis pathwayGenetic relatednessHigh expressionSevere clinical featuresSignificant economic burdenAnalysis of proteomesLabel-free quantification approachClostridium difficile PCR ribotypesDifferent PCR ribotypesBinary toxin genesDifficile PCR ribotypesBackgroundClostridium difficileToxic megacolonClinical featuresPseudomembranous colitisDifficile infectionFunctional annotationPathogenic roleFunctional complex