2012
Enrichment strategies for phosphoproteomics: state-of-the-art
Salovska B, Tichy A, Rezacova M, Vavrova J, Novotna E. Enrichment strategies for phosphoproteomics: state-of-the-art. Reviews In Analytical Chemistry 2012, 31: 29-41. DOI: 10.1515/revac-2011-0025.Peer-Reviewed Original ResearchProtein phosphorylationReversible post-translational modificationPost-translational modificationsAffinity enrichment techniquesPhosphorylated peptides/proteinsSubsequent mass spectrometry analysisAnalysis of phosphorylationEnrichment strategyDifferent enrichment strategiesTranslational regulationPhosphorylation sitesCellular signalingPhosphorylated proteinsMass spectrometry analysisPeptides/proteinsKey regulatorBiological processesChemical derivatizationLow abundancePhosphorylationMass spectrometryProteinSpectrometry analysisNonphosphorylated proteinsEnrichment technique
2011
Phosphoproteomics: Searching for a needle in a haystack
Tichy A, Salovska B, Rehulka P, Klimentova J, Vavrova J, Stulik J, Hernychova L. Phosphoproteomics: Searching for a needle in a haystack. Journal Of Proteomics 2011, 74: 2786-2797. PMID: 21839867, DOI: 10.1016/j.jprot.2011.07.018.Peer-Reviewed Original ResearchConceptsCharacterization of phosphoproteinsReversible phosphorylationCellular processesSignal transductionCell divisionNon-phosphorylated peptidesGene expressionInsufficient ionizationLow abundanceTryptic protein digestsMass spectrometryCritical roleProtein digestsEnrichment techniquePhosphoproteomePhosphoproteomicsPhosphoproteinPowerful toolTransductionPhosphorylationPhosphopeptidesProteinAbundanceApoptosisDifferentiation