1997
Reconstitution of calcium-regulated parathyroid hormone secretion from streptolysin-O-permeabilized parathyroid cells by guanosine 5'-O-(thio)triphosphate.
Matovcik L, Rhee S, Schaefer J, Kinder B. Reconstitution of calcium-regulated parathyroid hormone secretion from streptolysin-O-permeabilized parathyroid cells by guanosine 5'-O-(thio)triphosphate. Endocrinology 1997, 138: 1170-9. PMID: 9048624, DOI: 10.1210/endo.138.3.4971.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsCalciumCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesCattleCell Membrane PermeabilityCyclic AMPCytosolEnergy MetabolismGuanine NucleotidesGuanosine 5'-O-(3-Thiotriphosphate)Osmolar ConcentrationParathyroid GlandsParathyroid HormoneParotid GlandStreptolysinsTime FactorsConceptsPTH secretionParathyroid cellsIntracellular Ca2Hormone secretionParathyroid hormone secretionGuanosine 5'Dose-dependent mannerCalcium/calmodulin-dependent protein kinase IIPTH releaseCalmodulin-dependent protein kinase IIExtracellular Ca2Physiological intracellular Ca2Protein kinase IISecretionAmbient Ca2Low Ca2Ca2GTPγSCellsInverse relationshipCytosolic cofactorsKinase IIAddition of GTPγSAbsence of GTPγSDependent regulation
1987
Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas
Cohn J, Kinder B, Jamieson J, Delahunt N, Gorelick F. Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas. Biochimica Et Biophysica Acta 1987, 928: 320-331. PMID: 3105599, DOI: 10.1016/0167-4889(87)90192-3.Peer-Reviewed Original ResearchConceptsCalmodulin protein kinaseCalcium/calmodulin-dependent protein kinaseCalmodulin-dependent protein kinaseProtein kinaseMultifunctional calcium/calmodulin-dependent protein kinaseTwo-dimensional gel electrophoresisRibosomal proteinsThreonine residuesLarge subunitCellular functionsRibosomal substratesSubstrate specificityKinaseProteolytic degradationSubunitsGel electrophoresisHydrophobic chromatographyAffinity chromatographyPolyacrylamide gelsCalmodulinPurification procedureGel filtrationEnzyme preparationAutophosphorylationTissue