2022
TRPC1 channels underlie stretch-modulated sarcoplasmic reticulum calcium leak in cardiomyocytes
Streiff M, Corbin A, Ahmad A, Hunter C, Sachse F. TRPC1 channels underlie stretch-modulated sarcoplasmic reticulum calcium leak in cardiomyocytes. Frontiers In Physiology 2022, 13: 1056657. PMID: 36620209, PMCID: PMC9817106, DOI: 10.3389/fphys.2022.1056657.Peer-Reviewed Original ResearchNeonatal rat ventricular myocytesTransient receptor potentialTRPC1 channelsTRPC1 expressionCardiac diseaseReceptor potentialSarcoplasmic reticulum calcium leakAdenoviral transfectionRat ventricular myocytesSarco/endoplasmic reticulum CaPermeable ion channelsPhysiological roleSR leakPharmacological therapyEndoplasmic reticulum CaCalcium leakMechanosensitive modulationVentricular myocytesWestern blotReticulum CaRT-qPCRTRPC1CardiomyocytesDisease developmentSignificant reduction
2020
Modulation of Calcium Transients in Cardiomyocytes by Transient Receptor Potential Canonical 6 Channels
Ahmad A, Streiff M, Hunter C, Sachse F. Modulation of Calcium Transients in Cardiomyocytes by Transient Receptor Potential Canonical 6 Channels. Frontiers In Physiology 2020, 11: 44. PMID: 32116757, PMCID: PMC7033547, DOI: 10.3389/fphys.2020.00044.Peer-Reviewed Original ResearchNeonatal rat ventricular myocytesTRPC6 channelsCytosolic calcium concentrationCalcium loadTransient Receptor Potential Canonical 6 ChannelsCalcium transientsCalcium concentrationSarcoplasmic reticulum calcium loadCardiac hypertrophic remodelingActivated T cellsCalcineurin-nuclear factorEnhanced-green fluorescent proteinRat ventricular myocytesNon-selective cation channelsTransient receptor potentialCytosolic calcium transientsHypertrophic remodelingSustained elevationSR calcium loadT cellsIntracellular calciumCardiac hypertrophyCalcium dyeIndo-1Ventricular myocytesLocation and function of transient receptor potential canonical channel 1 in ventricular myocytes
Hu Q, Ahmad A, Seidel T, Hunter C, Streiff M, Nikolova L, Spitzer K, Sachse F. Location and function of transient receptor potential canonical channel 1 in ventricular myocytes. Journal Of Molecular And Cellular Cardiology 2020, 139: 113-123. PMID: 31982426, PMCID: PMC7085981, DOI: 10.1016/j.yjmcc.2020.01.008.Peer-Reviewed Original ResearchConceptsNeonatal rat ventricular myocytesRabbit ventricular myocytesVentricular myocytesSarcoplasmic reticulumChannel blocker SKF-96365TRPC1 overexpressionTransient receptor potential canonical channel 1Physiological pacing ratesTRPC1 channelsAdult rabbit ventricular myocytesRole of TRPC1Rat ventricular myocytesSarco/endoplasmic reticulum CaTransient receptor potentialMammalian ventricular myocytesIsolated rabbit cardiomyocytesEndoplasmic reticulum CaSKF-96365Free solutionCardiac hypertrophyPacing rateAction potentialsTRPC channelsTransverse tubular systemReticulum Ca