2023
A dual role for the chromatin reader ORCA/LRWD1 in targeting the origin recognition complex to chromatin
Sahu S, Ekundayo B, Kumar A, Bleichert F. A dual role for the chromatin reader ORCA/LRWD1 in targeting the origin recognition complex to chromatin. The EMBO Journal 2023, 42: embj2023114654. PMID: 37551430, PMCID: PMC10505921, DOI: 10.15252/embj.2023114654.Peer-Reviewed Original ResearchConceptsOrigin recognition complexH4K20 trimethylationHeterochromatin replicationRecognition complexCryo-electron microscopy structureLocal chromatin environmentSpecific histone marksSpecific chromatin contextsMcm2-7 loadingTernary complex assemblyChromatin environmentChromatin marksChromatin contextHistone marksHistone modificationsReplication initiationEukaryotic cellsMicroscopy structureChromatin condensatesORC recruitmentDNA replicationMammalian cellsNucleosomal DNAAromatic cageComplex assembly
2022
Plant-specific HDT family histone deacetylases are nucleoplasmins
Bobde R, Kumar A, Vasudevan D. Plant-specific HDT family histone deacetylases are nucleoplasmins. The Plant Cell 2022, 34: 4760-4777. PMID: 36069647, PMCID: PMC9709999, DOI: 10.1093/plcell/koac275.Peer-Reviewed Original ResearchConceptsN-terminal domainHistone chaperonesH2A/H2B dimersHistones H3/H4H3/H4Nucleosome dynamicsH2B dimersSequence similarityHistone acetyltransferaseEnigmatic familyHistone acetylationFunctional characterizationNucleoplasminGene expressionHistone deacetylasesC-terminalHistone deacetylaseUrea conditionsHistone oligomersSolution structureChaperonesDifferent familiesArabidopsis
2021
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays.
Bobde R, Saharan K, Baral S, Gandhi S, Samal A, Sundaram R, Kumar A, Singh A, Datta A, Vasudevan D. In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays. Journal Of Visualized Experiments 2021 PMID: 35037657, DOI: 10.3791/63218.Peer-Reviewed Original ResearchConceptsHistone chaperonesH3/H4 tetramersNucleosome assembly processH3/H4H2A/H2BCore histones H2APull-down assaysAnalytical size exclusion chromatographyEukaryotic chromatinH4 tetramersHistone H2AH2A/Core histonesHistone octamerSingle copyChaperonesHistonesCellular cytoplasmDiverse classChromatinProteinDNAH2BH4Non-specific interactions