2021
Biochemical characterization of an E. coli cell division factor FtsE shows ATPase cycles similar to the NBDs of ABC-transporters
Mallick S, Kumar A, Dodia H, Alexander C, Vasudevan D, Beuria T. Biochemical characterization of an E. coli cell division factor FtsE shows ATPase cycles similar to the NBDs of ABC-transporters. Bioscience Reports 2021, 41: bsr20203034. PMID: 33320186, PMCID: PMC7791547, DOI: 10.1042/bsr20203034.Peer-Reviewed Original ResearchConceptsFtsEX complexPG hydrolysisABC transportersCytoplasmic loopBiochemical characterizationATPase cycleCouple ATP hydrolysisATP-binding siteATPase activityE. coliBacterial cell wallTransduce signalsInner membraneSequence similarityBacterial divisionSignal transductionPeptidoglycan layerMid cellATP hydrolysisCell wallSoluble expressionBiochemical propertiesFtsE.Direct interactionTransport molecules
2019
Structural Characterization of Arabidopsis thaliana NAP1-Related Protein 2 (AtNRP2) and Comparison with Its Homolog AtNRP1
Kumar A, Singh A, Bobde R, Vasudevan D. Structural Characterization of Arabidopsis thaliana NAP1-Related Protein 2 (AtNRP2) and Comparison with Its Homolog AtNRP1. Molecules 2019, 24: 2258. PMID: 31213016, PMCID: PMC6630525, DOI: 10.3390/molecules24122258.Peer-Reviewed Original ResearchConceptsNucleosome assembly proteinHistone chaperonesFamily proteinsNAP familyIsothermal titration calorimetry experimentsTitration calorimetry experimentsAssembly proteinOligomerization statusPlant tissuesSequence identityDimerization helixSimilar foldSimilar proteinsBiophysical studiesSpecific functionsStructural superpositionProtein 2ProteinPlantsChaperonesElectrophoretic mobilityStructural studiesCrystal structureThermal melting experimentsStructural architecture