2022
A mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6
Schmidt JM, Yang R, Kumar A, Hunker O, Seebacher J, Bleichert F. A mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6. Nature Communications 2022, 13: 1059. PMID: 35217664, PMCID: PMC8881611, DOI: 10.1038/s41467-022-28695-w.Peer-Reviewed Original ResearchConceptsOrigin recognition complexS. cerevisiaeCyclin-dependent kinase phosphorylationMcm2-7 loadingN-terminal domainCryo-electron microscopyCDK phosphorylationRecognition complexDNA replicationReplication originsÅ resolutionKinase phosphorylationMechanism of originCdc6Coordinated actionCerevisiaePhosphorylationDNAInhibitory signalsStructural detailsSite regulationRecruitmentOrc6AssemblyCdt1
2019
Structural Characterization of Arabidopsis thaliana NAP1-Related Protein 2 (AtNRP2) and Comparison with Its Homolog AtNRP1
Kumar A, Singh A, Bobde R, Vasudevan D. Structural Characterization of Arabidopsis thaliana NAP1-Related Protein 2 (AtNRP2) and Comparison with Its Homolog AtNRP1. Molecules 2019, 24: 2258. PMID: 31213016, PMCID: PMC6630525, DOI: 10.3390/molecules24122258.Peer-Reviewed Original ResearchConceptsNucleosome assembly proteinHistone chaperonesFamily proteinsNAP familyIsothermal titration calorimetry experimentsTitration calorimetry experimentsAssembly proteinOligomerization statusPlant tissuesSequence identityDimerization helixSimilar foldSimilar proteinsBiophysical studiesSpecific functionsStructural superpositionProtein 2ProteinPlantsChaperonesElectrophoretic mobilityStructural studiesCrystal structureThermal melting experimentsStructural architecture