2021
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays.
Bobde R, Saharan K, Baral S, Gandhi S, Samal A, Sundaram R, Kumar A, Singh A, Datta A, Vasudevan D. In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays. Journal Of Visualized Experiments 2021 PMID: 35037657, DOI: 10.3791/63218.Peer-Reviewed Original ResearchConceptsHistone chaperonesH3/H4 tetramersNucleosome assembly processH3/H4H2A/H2BCore histones H2APull-down assaysAnalytical size exclusion chromatographyEukaryotic chromatinH4 tetramersHistone H2AH2A/Core histonesHistone octamerSingle copyChaperonesHistonesCellular cytoplasmDiverse classChromatinProteinDNAH2BH4Non-specific interactions
2019
Structural Characterization of Arabidopsis thaliana NAP1-Related Protein 2 (AtNRP2) and Comparison with Its Homolog AtNRP1
Kumar A, Singh A, Bobde R, Vasudevan D. Structural Characterization of Arabidopsis thaliana NAP1-Related Protein 2 (AtNRP2) and Comparison with Its Homolog AtNRP1. Molecules 2019, 24: 2258. PMID: 31213016, PMCID: PMC6630525, DOI: 10.3390/molecules24122258.Peer-Reviewed Original ResearchConceptsNucleosome assembly proteinHistone chaperonesFamily proteinsNAP familyIsothermal titration calorimetry experimentsTitration calorimetry experimentsAssembly proteinOligomerization statusPlant tissuesSequence identityDimerization helixSimilar foldSimilar proteinsBiophysical studiesSpecific functionsStructural superpositionProtein 2ProteinPlantsChaperonesElectrophoretic mobilityStructural studiesCrystal structureThermal melting experimentsStructural architecture