In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays.
Bobde R, Saharan K, Baral S, Gandhi S, Samal A, Sundaram R, Kumar A, Singh A, Datta A, Vasudevan D. In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays. Journal Of Visualized Experiments 2021 PMID: 35037657, DOI: 10.3791/63218.Peer-Reviewed Original ResearchConceptsHistone chaperonesH3/H4 tetramersNucleosome assembly processH3/H4H2A/H2BCore histones H2APull-down assaysAnalytical size exclusion chromatographyEukaryotic chromatinH4 tetramersHistone H2AH2A/Core histonesHistone octamerSingle copyChaperonesHistonesCellular cytoplasmDiverse classChromatinProteinDNAH2BH4Non-specific interactions