2018
Analysis of Cellular Tyrosine Phosphorylation via Chemical Rescue of Conditionally Active Abl Kinase
Wang Z, Kim MS, Martinez-Ferrando I, Koleske A, Pandey A, Cole P. Analysis of Cellular Tyrosine Phosphorylation via Chemical Rescue of Conditionally Active Abl Kinase. Biochemistry 2018, 57: 1390-1398. PMID: 29341593, PMCID: PMC5906802, DOI: 10.1021/acs.biochem.7b01158.Peer-Reviewed Original ResearchConceptsProtein kinaseNonreceptor tyrosine kinases AblMass spectrometry-based quantitative proteomicsNovel putative substratesTyrosine kinase AblCellular tyrosine phosphorylationExtracellular growth factorsChemical rescue approachIntracellular signal transductionQuantitative phosphoproteomicsUnanticipated functionCellular physiologyGrowth factorPhosphorylation sitesPutative substratesDirect substrateDownstream substratesSignal transductionReceptor kinaseQuantitative proteomicsTyrosine phosphorylationActive Abl kinasesAbl kinaseChemical rescueKinase
2013
WAVE2 Regulates Epithelial Morphology and Cadherin Isoform Switching through Regulation of Twist and Abl
Bryce NS, Reynolds AB, Koleske AJ, Weaver AM. WAVE2 Regulates Epithelial Morphology and Cadherin Isoform Switching through Regulation of Twist and Abl. PLOS ONE 2013, 8: e64533. PMID: 23691243, PMCID: PMC3654908, DOI: 10.1371/journal.pone.0064533.Peer-Reviewed Original ResearchConceptsEpithelial morphogenesisAbl kinaseActin cytoskeletal rearrangementCell-cell junctionsE-cadherinRegulation of TwistTotal cell lysatesAbl kinase activityEpithelial morphologyTranscription factor Twist1N-cadherin proteinTwist1 expressionWAVE2 complexABL substratesCellular signalingInhibition of AblCadherin isoformsCytoskeletal rearrangementsTyrosine phosphorylationEpithelial-mesenchymal transitionKinase activityMorphological phenotypesFilament localizationCadherin expressionWAVE2
2011
Cortactin phosphorylation regulates cell invasion through a pH-dependent pathway
Magalhaes MA, Larson DR, Mader CC, Bravo-Cordero JJ, Gil-Henn H, Oser M, Chen X, Koleske AJ, Condeelis J. Cortactin phosphorylation regulates cell invasion through a pH-dependent pathway. Journal Of Cell Biology 2011, 195: 903-920. PMID: 22105349, PMCID: PMC3257566, DOI: 10.1083/jcb.201103045.Peer-Reviewed Original ResearchMeSH KeywordsActin Depolymerizing FactorsAdaptor Proteins, Signal TransducingCation Transport ProteinsCell Line, TumorCell Surface ExtensionsCortactinHumansHydrogen-Ion ConcentrationModels, BiologicalNeoplasm InvasivenessOncogene ProteinsPhosphorylationSodium-Hydrogen Exchanger 1Sodium-Hydrogen ExchangersConceptsCortactin phosphorylationCell invasionInvadopodia maturationCortactin tyrosine phosphorylationPH-dependent regulationInvasive protrusionsPH-dependent pathwayCofilin activityCofilin regulationTyrosine phosphorylationExchanger NHE1Cofilin activationPhosphorylationInvadopodiaProteolytic activityPrecise mechanismInvasionNHE1RegulationDynamic cyclePathwayMaturationTumor cellsNck1Cofilin
2010
Synaptic Clustering of PSD-95 Is Regulated by c-Abl through Tyrosine Phosphorylation
de Arce K, Varela-Nallar L, Farias O, Cifuentes A, Bull P, Couch BA, Koleske AJ, Inestrosa NC, Alvarez AR. Synaptic Clustering of PSD-95 Is Regulated by c-Abl through Tyrosine Phosphorylation. Journal Of Neuroscience 2010, 30: 3728-3738. PMID: 20220006, PMCID: PMC2872795, DOI: 10.1523/jneurosci.2024-09.2010.Peer-Reviewed Original ResearchConceptsPSD-95Protein postsynaptic density protein 95Postsynaptic density protein 95PSD-95 clusteringHippocampal neuron culturesFirst postnatal weekC-AblC-Abl levelsPresynaptic markersTyrosine phosphorylationRat hippocampusPostnatal weekPostsynaptic sitesSynaptic clusteringNeuron culturesSynaptic functionC-Abl kinase activityReduced synapsesSynapse formationPostsynaptic compartmentsBrain synapsesGenetic inhibitionSynapsesTyrosine kinaseC-Abl tyrosine kinase
2008
The c-Abl tyrosine kinase regulates actin remodeling at the immune synapse
Huang Y, Comiskey EO, Dupree RS, Li S, Koleske AJ, Burkhardt JK. The c-Abl tyrosine kinase regulates actin remodeling at the immune synapse. Blood 2008, 112: 111-119. PMID: 18305217, PMCID: PMC2435682, DOI: 10.1182/blood-2007-10-118232.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdaptor Proteins, Signal TransducingAnimalsCells, CulturedHumansInterleukin-2Jurkat CellsLymphocyte ActivationMiceMice, KnockoutPhosphorylationProtein BindingProteinsProto-Oncogene Proteins c-ablPseudopodiaReceptors, Antigen, T-CellSignal TransductionT-LymphocytesTranscription, GeneticWiskott-Aldrich Syndrome Protein FamilyConceptsC-AblImmune synapseActin responseC-Abl nonreceptor tyrosine kinaseT cell activationTyrosine kinaseC-Abl tyrosine kinaseC-Abl bindsActin regulatory proteinsNonreceptor tyrosine kinaseChemokine-induced T cell migrationT cell receptor engagementSH2 domainActin dynamicsActin reorganizationTyrosine phosphorylationRegulatory proteinsActin polymerizationDownstream eventsNormal localizationConditional knockout miceCoordinate actionReceptor engagementKinaseLamellipodial spreading
2007
Use of a Chemical Genetic Technique To Identify Myosin IIB as a Substrate of the Abl-Related Gene (Arg) Tyrosine Kinase
Boyle SN, Koleske AJ. Use of a Chemical Genetic Technique To Identify Myosin IIB as a Substrate of the Abl-Related Gene (Arg) Tyrosine Kinase. Biochemistry 2007, 46: 11614-11620. PMID: 17892306, DOI: 10.1021/bi701119s.Peer-Reviewed Original ResearchConceptsPutative substratesMyosin IIBGene Tyrosine KinaseUnnatural ATP analoguesProtein substrate specificityPhosphotyrosine-containing proteinsSer/ThrAbl/ArgChemical-genetic techniqueCell morphogenesisKinase substrateDirect substrateFamily kinasesTyrosine phosphorylationSubstrate specificityGenetic techniquesNucleotide specificityMolecular mechanismsTyrosine kinaseK-252aUnexpected high levelATP analogGene kinaseKinaseABL
2004
Abl-dependent tyrosine phosphorylation of Sos-1 mediates growth-factor-induced Rac activation
Sini P, Cannas A, Koleske AJ, Di Fiore PP, Scita G. Abl-dependent tyrosine phosphorylation of Sos-1 mediates growth-factor-induced Rac activation. Nature Cell Biology 2004, 6: 268-274. PMID: 15039778, DOI: 10.1038/ncb1096.Peer-Reviewed Original ResearchConceptsGuanine nucleotide exchange factorsSos-1Rac activationTyrosine phosphorylationFunction of RacNon-receptor tyrosine kinase AblActin cytoskeleton remodellingNucleotide exchange factorsRac-GEF activityTyrosine kinase AblActivation of RTKsActivity of RacReceptor tyrosine kinasesCytoskeleton remodellingActin remodellingSignal transductionMolecular connectionMolecular mechanismsTyrosine kinaseBcr-Abl oncoproteinRacPharmacological interferencePhosphorylationGrowth factorABL signals