1995
Association of an activator with an RNA polymerase II holoenzyme.
Hengartner CJ, Thompson CM, Zhang J, Chao DM, Liao SM, Koleske AJ, Okamura S, Young RA. Association of an activator with an RNA polymerase II holoenzyme. Genes & Development 1995, 9: 897-910. PMID: 7774808, DOI: 10.1101/gad.9.8.897.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceGenes, FungalHerpes Simplex Virus Protein Vmw65Macromolecular SubstancesMediator ComplexModels, GeneticMolecular Sequence DataMutationPrecipitin TestsProtein BindingRestriction MappingRNA Polymerase IISaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Analysis, DNASuppression, GeneticTranscription FactorsTranscription, GeneticConceptsRNA polymerase II holoenzymeRNA polymerase IISRB proteinsPolymerase IIDomain of VP16General transcription factorsDirect interactionTranscription apparatusTranscriptional activatorMediators of activationTranscription initiationTranscription factorsImmunoprecipitation experimentsHoloenzymeGenesActivatorAffinity columnProteinSubcomplexPromoterVP16BindsInteractionHallmarkActivation
1994
An RNA polymerase II holoenzyme responsive to activators
Koleske A, Young R. An RNA polymerase II holoenzyme responsive to activators. Nature 1994, 368: 466-469. PMID: 8133894, DOI: 10.1038/368466a0.Peer-Reviewed Original ResearchConceptsRNA polymerase IIGeneral transcription factorsRNA polymerase II holoenzymePolymerase IITranscription factorsPromoter DNAMulti-component complexesGAL4-VP16Initiation factorsRegulatory proteinsHoloenzymeProteinDNAOrdered fashionCerevisiaeTranscriptionVivoPromoterActivatorAssemblyEquimolar amountsComplexes