2016
Phosphatidylserine exposure is required for ADAM17 sheddase function
Sommer A, Kordowski F, Büch J, Maretzky T, Evers A, Andrä J, Düsterhöft S, Michalek M, Lorenzen I, Somasundaram P, Tholey A, Sönnichsen F, Kunzelmann K, Heinbockel L, Nehls C, Gutsmann T, Grötzinger J, Bhakdi S, Reiss K. Phosphatidylserine exposure is required for ADAM17 sheddase function. Nature Communications 2016, 7: 11523. PMID: 27161080, PMCID: PMC4866515, DOI: 10.1038/ncomms11523.Peer-Reviewed Original ResearchConceptsMembrane-proximal domainVital cellular functionsSheddase functionTransmembrane substratesCellular functionsScott syndromeSheddase activityProximal domainPhosphatidylserine exposureSurface-exposed phosphatidylserinePS exposurePhosphatidylserineProminent memberProteaseCellsRaji cellsCleavageSurface exposureDomain
2012
Melittin Modulates Keratinocyte Function through P2 Receptor-dependent ADAM Activation*
Sommer A, Fries A, Cornelsen I, Speck N, Koch-Nolte F, Gimpl G, Andrä J, Bhakdi S, Reiss K. Melittin Modulates Keratinocyte Function through P2 Receptor-dependent ADAM Activation*. Journal Of Biological Chemistry 2012, 287: 23678-23689. PMID: 22613720, PMCID: PMC3390642, DOI: 10.1074/jbc.m112.362756.Peer-Reviewed Original ResearchMeSH KeywordsADAM ProteinsADAM10 ProteinADAM17 ProteinAdenosine TriphosphateAmyloid Precursor Protein SecretasesAnimalsBlotting, WesternCadherinsCell LineCell SurvivalCells, CulturedDose-Response Relationship, DrugEmbryo, MammalianErbB ReceptorsExtracellular Signal-Regulated MAP KinasesFibroblastsHEK293 CellsHumansKeratinocytesMelittenMembrane ProteinsMiceMice, KnockoutModels, BiologicalPhosphorylationReceptors, Purinergic P2X7Reverse Transcriptase Polymerase Chain ReactionConceptsAnti-cancer agentsP2 receptorsADAM activationP2 receptor antagonistsRelease of TGFMultiple cellular functionsPurinergic P2 receptorsAdhesion molecule E-cadherinE-cadherinReceptor antagonistP2X7 receptorHuman HaCaT keratinocytesCellular functionsPresence of ATPasesDownstream eventsATP releaseEGFR phosphorylationHuman neutrophilsEGF receptorEndothelial cellsERK1/2 phosphorylationKeratinocyte proliferationReceptorsBee venomCell migration