2019
Mutagenesis of the ADAM17-phosphatidylserine–binding motif leads to embryonic lethality in mice
Veit M, Ahrens B, Seidel J, Sommer A, Bhakdi S, Reiss K. Mutagenesis of the ADAM17-phosphatidylserine–binding motif leads to embryonic lethality in mice. Life Science Alliance 2019, 2: e201900430. PMID: 31455669, PMCID: PMC6712283, DOI: 10.26508/lsa.201900430.Peer-Reviewed Original ResearchConceptsEmbryonic lethalityVital cellular functionsAnionic phospholipid headgroupsTransmembrane substratesCellular functionsProtease functionBinding motifAmino acidsCell surfaceMutant proteasePrimary hepatocytesMotifMutagenesisPhosphatidylserinePhospholipid headgroupsProminent memberCentral roleEssential triggerLethalitySurface exposureOncogenesisProteaseADAM17DisintegrinMice
2018
Anoctamin-6 regulates ADAM sheddase function
Veit M, Koyro K, Ahrens B, Bleibaum F, Munz M, Rövekamp H, Andrä J, Schreiber R, Kunzelmann K, Sommer A, Bhakdi S, Reiss K. Anoctamin-6 regulates ADAM sheddase function. Biochimica Et Biophysica Acta (BBA) - Molecular Cell Research 2018, 1865: 1598-1610. PMID: 30327201, DOI: 10.1016/j.bbamcr.2018.08.011.Peer-Reviewed Original ResearchConceptsAnoctamin 6Scramblase activityHuman umbilical vein endothelial cellsTNF receptor 1Vital cellular functionsPhospholipid scramblase activityPS exposureHEK293T cellsTGF-alphaTransformation of cellsSheddase functionTransmembrane substratesCellular functionsADAM17 substratesPlasma membraneUmbilical vein endothelial cellsInhibitor experimentsSheddase activitySubstrate cleavageImportant regulatorVein endothelial cellsActivator PMAProminent memberAmphiregulinEndothelial cells
2016
Phosphatidylserine exposure is required for ADAM17 sheddase function
Sommer A, Kordowski F, Büch J, Maretzky T, Evers A, Andrä J, Düsterhöft S, Michalek M, Lorenzen I, Somasundaram P, Tholey A, Sönnichsen F, Kunzelmann K, Heinbockel L, Nehls C, Gutsmann T, Grötzinger J, Bhakdi S, Reiss K. Phosphatidylserine exposure is required for ADAM17 sheddase function. Nature Communications 2016, 7: 11523. PMID: 27161080, PMCID: PMC4866515, DOI: 10.1038/ncomms11523.Peer-Reviewed Original ResearchConceptsMembrane-proximal domainVital cellular functionsSheddase functionTransmembrane substratesCellular functionsScott syndromeSheddase activityProximal domainPhosphatidylserine exposureSurface-exposed phosphatidylserinePS exposurePhosphatidylserineProminent memberProteaseCellsRaji cellsCleavageSurface exposureDomain