2018
Anoctamin-6 regulates ADAM sheddase function
Veit M, Koyro K, Ahrens B, Bleibaum F, Munz M, Rövekamp H, Andrä J, Schreiber R, Kunzelmann K, Sommer A, Bhakdi S, Reiss K. Anoctamin-6 regulates ADAM sheddase function. Biochimica Et Biophysica Acta (BBA) - Molecular Cell Research 2018, 1865: 1598-1610. PMID: 30327201, DOI: 10.1016/j.bbamcr.2018.08.011.Peer-Reviewed Original ResearchConceptsAnoctamin 6Scramblase activityHuman umbilical vein endothelial cellsTNF receptor 1Vital cellular functionsPhospholipid scramblase activityPS exposureHEK293T cellsTGF-alphaTransformation of cellsSheddase functionTransmembrane substratesCellular functionsADAM17 substratesPlasma membraneUmbilical vein endothelial cellsInhibitor experimentsSheddase activitySubstrate cleavageImportant regulatorVein endothelial cellsActivator PMAProminent memberAmphiregulinEndothelial cells
2017
Extracellular sphingomyelinase activity impairs TNF-α-induced endothelial cell death via ADAM17 activation and TNF receptor 1 shedding
Sommer A, Düppe M, Baumecker L, Kordowski F, Büch J, Chico J, Fritsch J, Schütze S, Adam D, Sperrhacke M, Bhakdi S, Reiss K. Extracellular sphingomyelinase activity impairs TNF-α-induced endothelial cell death via ADAM17 activation and TNF receptor 1 shedding. Oncotarget 2017, 5: 72584-72596. PMID: 29069811, PMCID: PMC5641154, DOI: 10.18632/oncotarget.19983.Peer-Reviewed Original ResearchHuman umbilical vein endothelial cellsEndothelial cell deathCell deathTNF-α receptor 1COS7 cellsVital cellular functionsPhospholipid scramblasesSheddase functionCellular functionsEffect of SMaseADAM activityExtracellular sphingomyelinaseSignaling cascadesEndothelial cellsUmbilical vein endothelial cellsReceptor 1Phospholipid asymmetryPS externalizationSheddase activityCell survivalImportant regulatorVein endothelial cellsCell typesEndothelial cell functionEndothelial cell proliferation
2016
Phosphatidylserine exposure is required for ADAM17 sheddase function
Sommer A, Kordowski F, Büch J, Maretzky T, Evers A, Andrä J, Düsterhöft S, Michalek M, Lorenzen I, Somasundaram P, Tholey A, Sönnichsen F, Kunzelmann K, Heinbockel L, Nehls C, Gutsmann T, Grötzinger J, Bhakdi S, Reiss K. Phosphatidylserine exposure is required for ADAM17 sheddase function. Nature Communications 2016, 7: 11523. PMID: 27161080, PMCID: PMC4866515, DOI: 10.1038/ncomms11523.Peer-Reviewed Original ResearchConceptsMembrane-proximal domainVital cellular functionsSheddase functionTransmembrane substratesCellular functionsScott syndromeSheddase activityProximal domainPhosphatidylserine exposureSurface-exposed phosphatidylserinePS exposurePhosphatidylserineProminent memberProteaseCellsRaji cellsCleavageSurface exposureDomain