2024
A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva.
Peno C, Lin T, Hislop M, Yolda-Carr D, Farjado K, York A, Pitzer V, Weinberger D, Bei A, Allicock O, Wyllie A. A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva. Microbiology Spectrum 2024, e0059124. PMID: 39028185, DOI: 10.1128/spectrum.00591-24.Peer-Reviewed Original ResearchDetection of pneumococciDetection of pneumococcal carriagePneumococcal carriageCarriage surveillanceLow-resource settingsChildren attending childcare centersCarriage of pneumococciDNA extractionSaliva samplesMolecular methodsCultural enrichmentImprove surveillance effortsQPCR-based protocolPneumococcal vaccineExtraction-free methodMolecular detectionNucleic acid extractionVaccination strategiesPneumococciCulture-enrichment methodExtraction-free protocolPurified DNASalivaPaired samplesCarriage
2022
Evaluation of the Liberty16 Mobile Real Time PCR Device for Use With the SalivaDirect Assay for SARS-CoV-2 Testing
Yolda-Carr D, Thammavongsa DA, Vega N, Turner SJ, Pickering PJ, Wyllie AL. Evaluation of the Liberty16 Mobile Real Time PCR Device for Use With the SalivaDirect Assay for SARS-CoV-2 Testing. Frontiers In Cellular And Infection Microbiology 2022, 11: 808773. PMID: 35118013, PMCID: PMC8804088, DOI: 10.3389/fcimb.2021.808773.Peer-Reviewed Original ResearchConceptsLimit of detectionReal-time PCR deviceSARS-CoV-2 testingSmall portable devicesPCR devicePortable devicesSARS-CoV-2 detectionHigh sensitivitySARS-CoV-2 RNADevicesInfectious respiratory diseaseSARS-CoV-2Low-resource settingsRespiratory diseaseImplementation of testingFurther optimizationSaliva samplesProtocolGold standardRT-qPCRTimely accessCOVID-19 pandemicDetectionLOD rangeSensitivity