2000
Prostaglandin E2 interaction with AVP: effects on AQP2 phosphorylation and distribution
Zelenina M, Christensen B, Palmér J, Nairn A, Nielsen S, Aperia A. Prostaglandin E2 interaction with AVP: effects on AQP2 phosphorylation and distribution. American Journal Of Physiology. Renal Physiology 2000, 278: f388-f394. PMID: 10710543, DOI: 10.1152/ajprenal.2000.278.3.f388.Peer-Reviewed Original ResearchConceptsTranslocation of AQP2AQP2 phosphorylationPlasma membraneAquaporin-2Subcellular distributionPlasma membrane-enriched fractionVesicle-enriched fractionsMembrane-enriched fractionDuct water permeabilityConsensus sitesIntracellular vesiclesPhosphorylationDifferential centrifugation techniqueAction of arginineRenal inner medullaE2 interactionRat renal inner medullaTranslocationInner medullaDose-dependent mannerWater channelsMembraneDephosphorylationTraffickingProtein
1999
Arginine vasopressin stimulates phosphorylation of aquaporin-2 in rat renal tissue
Nishimoto G, Zelenina M, Li D, Yasui M, Aperia A, Nielsen S, Nairn A. Arginine vasopressin stimulates phosphorylation of aquaporin-2 in rat renal tissue. American Journal Of Physiology 1999, 276: f254-f259. PMID: 9950956, DOI: 10.1152/ajprenal.1999.276.2.f254.Peer-Reviewed Original ResearchConceptsPhosphorylation of AQP2Protein kinase AAquaporin-2Two-dimensional phosphopeptide mappingCAMP-dependent protein kinase AConsensus phosphorylation sitesActivation of PKAPhosphopeptide mappingPhosphorylation sitesMaximal phosphorylationAQP2 phosphorylationKinase APhosphorylationSer256Immunoblot analysis