1996
Inhibition of Tumor Necrosis Factor Signal Transduction in Endothelial Cells by Dimethylaminopurine*
Marino M, Dunbar J, Wu L, Ngaiza J, Han H, Guo D, Matsushita M, Nairn A, Zhang Y, Kolesnick R, Jaffe E, Donner D. Inhibition of Tumor Necrosis Factor Signal Transduction in Endothelial Cells by Dimethylaminopurine*. Journal Of Biological Chemistry 1996, 271: 28624-28629. PMID: 8910494, DOI: 10.1074/jbc.271.45.28624.Peer-Reviewed Original ResearchMeSH KeywordsAdenineAnimalsCattleEndothelium, VascularEnzyme InhibitorsEukaryotic Initiation Factor-4EHistaminePeptide Elongation Factor 2Peptide Elongation FactorsPeptide Initiation FactorsPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene Proteins c-rafSignal TransductionTumor Necrosis Factor-alphaConceptsBovine aortic endothelial cellsElongation factor 2Distinct signal transduction cascadesEukaryotic initiation factor 4ETNF signal transduction pathwayEF-2 phosphorylationC-Jun N-terminal kinaseSignal transduction cascadeInitiation factor 4EProtein kinase activitySignal transduction pathwaysEndothelial cellsN-terminal kinaseTNF actionPhosphorylation cascadeEIF-4ESignal transductionTransduction cascadeTransduction pathwaysResponse of BAECsJun-B expressionKinase activityProtein synthesisPhosphorylationCell types
1990
Amiloride analogs induce the phosphorylation of elongation factor-2 in vascular endothelial cells.
Demolle D, Lecomte M, Boutherin-Falson O, Cragoe E, Nairn A, Boeynaems J. Amiloride analogs induce the phosphorylation of elongation factor-2 in vascular endothelial cells. Molecular Pharmacology 1990, 37: 827-32. PMID: 2359404.Peer-Reviewed Original ResearchConceptsElongation factor 2Protein synthesisFactor 2Rabbit reticulocyte lysateCell-free systemBovine aortic endothelial cellsDependent phosphorylationReticulocyte lysateEndothelial cellsAmiloride analoguesPhosphorylationSimilar MrCytosolic pHVascular endothelial cellsProteinAnalogues of amilorideAortic endothelial cellsPotent inhibitorInhibitory effectAntiportCellsEIPAAmilorideATPLysates
1989
Thrombin and Histamine Stimulate the Phosphorylation of Elongation Factor 2 in Human Umbilical Vein Endothelial Cells
Mackie K, Nairn A, Hampel G, Lam G, Jaffe E. Thrombin and Histamine Stimulate the Phosphorylation of Elongation Factor 2 in Human Umbilical Vein Endothelial Cells. Journal Of Biological Chemistry 1989, 264: 1748-1753. PMID: 2536373, DOI: 10.1016/s0021-9258(18)94250-x.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsElongation factor 2EF-2Calmodulin-dependent protein kinase IIIUmbilical vein endothelial cellsProtein synthesisVein endothelial cellsProtein kinase IIIPhosphoamino acid analysisFactor 2Kinase IIICell protein synthesisProtein phosphorylationCultured human umbilical vein endothelial cellsEndothelial cellsPeptidyl-tRNAPhosphorylationBiochemical studiesRapid transient increaseAcid analysisPhorbol dibutyrateIncubation of HUVECsIntracellular calcium levelsProteinEffect of thrombin