1992
Characterization of the cystic fibrosis transmembrane conductance regulator in a colonocyte cell line.
Cohn J, Nairn A, Marino C, Melhus O, Kole J. Characterization of the cystic fibrosis transmembrane conductance regulator in a colonocyte cell line. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 2340-2344. PMID: 1372442, PMCID: PMC48653, DOI: 10.1073/pnas.89.6.2340.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorConductance regulatorTwo-dimensional phosphopeptide mappingT84 cellsProtein kinase ACell linesProtein kinase CSDS/PAGEPhosphopeptide mappingPhosphorylation sitesProminent substrateCFTR peptidesEquivalent proteinsKinase ASame proteinKinase CTerminal sequenceCell lysatesN-glycanaseProteinAnti-peptide antibodiesImmunoblot signalsCFTR immunoreactivity
1990
Activation of protein kinase C results in the displacement of its myristoylated, alanine-rich substrate from punctate structures in macrophage filopodia.
Rosen A, Keenan K, Thelen M, Nairn A, Aderem A. Activation of protein kinase C results in the displacement of its myristoylated, alanine-rich substrate from punctate structures in macrophage filopodia. Journal Of Experimental Medicine 1990, 172: 1211-1215. PMID: 2212950, PMCID: PMC2188604, DOI: 10.1084/jem.172.4.1211.Peer-Reviewed Original ResearchConceptsProtein kinase CPKC-dependent phosphorylationPhosphorylation-dependent releaseProtein kinase C resultsAlanine-rich C kinase substrateDiverse cellular processesC kinase substrateCell-substratum interfacePhorbol esters resultsActivation of PKCPunctate stainingKinase substrateCellular processesProminent substratePunctate structuresMembrane cytoskeletonLoss of filopodiaPlasma membranePunctate distributionVariety of cellsCell spreadingMARCKSKinase CMacrophage filopodiaFilopodia