Thrombin and Histamine Stimulate the Phosphorylation of Elongation Factor 2 in Human Umbilical Vein Endothelial Cells
Mackie K, Nairn A, Hampel G, Lam G, Jaffe E. Thrombin and Histamine Stimulate the Phosphorylation of Elongation Factor 2 in Human Umbilical Vein Endothelial Cells. Journal Of Biological Chemistry 1989, 264: 1748-1753. PMID: 2536373, DOI: 10.1016/s0021-9258(18)94250-x.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsElongation factor 2EF-2Calmodulin-dependent protein kinase IIIUmbilical vein endothelial cellsProtein synthesisVein endothelial cellsProtein kinase IIIPhosphoamino acid analysisFactor 2Kinase IIICell protein synthesisProtein phosphorylationCultured human umbilical vein endothelial cellsEndothelial cellsPeptidyl-tRNAPhosphorylationBiochemical studiesRapid transient increaseAcid analysisPhorbol dibutyrateIncubation of HUVECsIntracellular calcium levelsProteinEffect of thrombin