Actions of Genistein on Cystic Fibrosis Transmembrane Conductance Regulator Channel Gating
Wang F, Zeltwanger S, Yang I, Nairn A, Hwang T. Actions of Genistein on Cystic Fibrosis Transmembrane Conductance Regulator Channel Gating. The Journal Of General Physiology 1998, 111: 477-490. PMID: 9482713, PMCID: PMC2217116, DOI: 10.1085/jgp.111.3.477.Peer-Reviewed Original ResearchConceptsCystic Fibrosis Transmembrane Conductance Regulator Channel GatingCFTR channelsSerine/threonine proteinTyrosine kinaseCystic fibrosis transmembrane conductance regulator (CFTR) channel activityDirect bindingHi-5 insect cellsCFTR channel currentsTyrosine phosphatase inhibitorMicroM genisteinProtein kinase AEffects of genisteinNonhydrolyzable ATP analogRecombinant CFTRProtein phosphatasePossible molecular mechanismsCFTR gatingInsect cellsPhosphatase inhibitorCalyculin ACFTR proteinAbsence of genisteinATP hydrolysisKinase ANIH3T3 cells