1990
Nerve Growth Factor‐Induced Down‐Regulation of Calmodulin‐Dependent Protein Kinase III in PC12 Cells Involves Cyclic AMP‐Dependent Protein Kinase
Brady M, Nairn A, Wagner J, Palfrey H. Nerve Growth Factor‐Induced Down‐Regulation of Calmodulin‐Dependent Protein Kinase III in PC12 Cells Involves Cyclic AMP‐Dependent Protein Kinase. Journal Of Neurochemistry 1990, 54: 1034-1039. PMID: 1689374, DOI: 10.1111/j.1471-4159.1990.tb02354.x.Peer-Reviewed Original ResearchConceptsWild-type cellsCalmodulin-dependent protein kinase IIICAMP-dependent protein kinase activityProtein kinase IIIProtein kinase activityEpidermal growth factorKinase IIIKinase activityA126-1B2 cellsCyclic AMP-dependent protein kinaseAMP-dependent protein kinasePC12 cellsNerve growth factorMutant PC12 cell lineElongation factor 2Growth factorAbility of NGFPC12 cell lineEffects of NGFProtein kinaseNGF additionA126-1B2Down regulationCell linesFactor 2
1987
The cyclic nucleotide-dependent phosphorylation of aortic smooth muscle membrane proteins
Parks T, Nairn A, Greengard P, Jamieson J. The cyclic nucleotide-dependent phosphorylation of aortic smooth muscle membrane proteins. Archives Of Biochemistry And Biophysics 1987, 255: 361-371. PMID: 3036005, DOI: 10.1016/0003-9861(87)90404-8.Peer-Reviewed Original ResearchConceptsG-kinaseA-kinaseGs proteinMembrane proteinsCyclic nucleotide-dependent phosphorylationCAMP-dependent protein kinase activityCGMP-dependent protein kinaseKinase catalytic subunitEndogenous A-kinaseProtein kinase activityPeptide mappingTwo-dimensional peptide mappingMembrane-bound formCyclic nucleotidesHigh-salt washMuscle membrane proteinsCatalytic subunitFunctional homologyProtein kinasePhosphorylation stateCytosolic formKinase activitySalt washIntracellular concentrationSoluble form