2020
DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells
Williams ND, Landajuela A, Kasula RK, Zhou W, Powell JT, Xi Z, Isaacs FJ, Berro J, Toomre D, Karatekin E, Lin C. DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells. Nano Letters 2020, 20: 8890-8896. PMID: 33164530, PMCID: PMC7726105, DOI: 10.1021/acs.nanolett.0c03925.Peer-Reviewed Original ResearchConceptsCopies of proteinsBrightness standardsFluorescence microscopyProtein countingMammalian cellsFluorescent proteinCopy numberLive cellsConventional quantification techniquesBiological LaboratoryFluorescence microscopeProteinDNA origamiVersatile toolCellsBiologyCurrent quantification methodsCopiesOrganic dyes
2015
Minimalist Model Systems Reveal Similarities and Differences between Membrane Interaction Modes of MCL1 and BAK*
Landeta O, Landajuela A, Garcia-Saez A, Basañez G. Minimalist Model Systems Reveal Similarities and Differences between Membrane Interaction Modes of MCL1 and BAK*. Journal Of Biological Chemistry 2015, 290: 17004-17019. PMID: 25987560, PMCID: PMC4505444, DOI: 10.1074/jbc.m114.602193.Peer-Reviewed Original ResearchConceptsBCL2 family proteinsFamily proteinsStructural foldMembrane environmentStable heterodimeric complexMembrane association modesFluorescence cross-correlation spectroscopyBCL2 family membersMembrane interaction modesCross-correlation spectroscopyCell fateMembrane associationHeterodimeric complexBCL2 familyMitochondrial membraneCellular factorsFluorescence-based techniquesMode of actionApoptosis-related factorsBakKey modulatorFRET measurementsMCL1ProteinCardiolipin content
2013
Proapoptotic Bax and Bak Proteins Form Stable Protein-permeable Pores of Tunable Size
Bleicken S, Landeta O, Landajuela A, Basañez G, García-Sáez A. Proapoptotic Bax and Bak Proteins Form Stable Protein-permeable Pores of Tunable Size. Journal Of Biological Chemistry 2013, 288: 33241-33252. PMID: 24100034, PMCID: PMC3829170, DOI: 10.1074/jbc.m113.512087.Peer-Reviewed Original ResearchConceptsMitochondrial outer membraneApoptotic poreOuter membraneMitochondrial apoptotic factorsSingle vesicle levelActivity of BaxProapoptotic protein BaxProteinaceous channelsVesicle levelMembrane permeabilizationBak proteinForm poresProtein BaxProapoptotic BaxCardiolipin concentrationCytochrome cApoptotic factorsBaxBakPermeabilizationProteinSimilar mechanismMembraneMembrane poresProtein concentration